Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-CD133抗体[EPR20980-104] - BSA and Azide free (ab226484)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
  • Western blot - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
  • Flow Cytometry - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
  • Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
  • Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20980-104] to CD133 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC PE Unconjugated

概述

  • 产品名称

    Anti-CD133抗体[EPR20980-104] - BSA and Azide free
    参阅全部 CD133 一抗

  • 描述

    兔单克隆抗体[EPR20980-104] to CD133 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, Flow Cyt, IPmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Caco-2 and NCCIT whole cell lysate. Human fetal kidney lysate. IHC-P: Human mammary gland, kidney, pancreas and pancreatic carcinoma tissues. Flow Cytometry: HEK-293T cells. IP: Caco-2 whole cell lysate.

  • 常规说明

    ab226484 is the carrier-free version of ab216323.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226484于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

靶标

  • 功能

    May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).

  • 组织特异性

    Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).

  • 疾病相关

    Retinitis pigmentosa 41
    Cone-rod dystrophy 12
    Stargardt disease 4
    Retinal macular dystrophy 2

  • 序列相似性

    Belongs to the prominin family.

  • 翻译后修饰

    Isoform 1 and isoform 2 are glycosylated.
    Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.

  • 细胞定位

    Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AC133 antibody
      • Antigen AC133 antibody
      • CD133 antibody
      • CORD12 antibody
      • Hematopoietic stem cell antigen antibody
      • hProminin antibody
      • MCDR2 antibody
      • MSTP061 antibody
      • OTTHUMP00000217744 antibody
      • OTTHUMP00000217745 antibody
      • OTTHUMP00000217746 antibody
      • PROM1 antibody
      • PROM1_HUMAN antibody
      • Prominin I antibody
      • Prominin like 1 antibody
      • Prominin like protein 1 precursor antibody
      • Prominin mouse like 1 antibody
      • Prominin-1 antibody
      • Prominin-like protein 1 antibody
      • Prominin1 antibody
      • PROML1 antibody
      • RP41 antibody
      • STGD4 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)

      Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labelling CD133 with ab216323 at 1/1000 dilution (1.386 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at Ready to use dilution. Positive staining on human pancreatic carcinoma. The section was incubated with ab216323 at 4°C overnight. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at Ready to use dilution.

      Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216323).

    • Western blot - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      Western blot - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      All lanes : Anti-CD133 antibody [EPR20980-104] (ab216323) at 1/1000 dilution

      Lane 1 : Caco-2 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 97 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?


      Exposure time: 8 minutes


      ab216323 was shown to react with CD133 in western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab216323 overnight at 4°C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with ECL reagent and imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)

      Immunohistochemical analysis of paraffin-embedded human mammary gland tissue labeling CD133 with ab216323 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human mammary gland (PMID: 18261235) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216323).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD133 with ab216323 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on parietal layer of Bowman’s capsule of human kidney (PMID: 19092120) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216323).

    • Flow Cytometry - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      Flow Cytometry - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)

      Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell)(Left) / Caco-2 (human colorectal adenocarcinoma epithelial cell)(Right) cell line labeling CD133 with ab216323 at 1/100 (Red) compared with Isotype Control Rabbit monoclonal IgG (ab172730) (BLack) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

      Negative control: 293T (PMID 20167130). Total viable cells were gated for the FC image.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216323).

    • Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)

      CD133 was immunoprecipitated from 0.35 mg Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab216323 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab216323 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
      Lane 1: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).
      Lane 2: ab216323 IP in Caco-2 whole cell lysate (+).
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of  ab216323 in Caco-2 whole cell lysate (-).
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216323).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab216323 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human pancreas, and negative on Langerhans cells of the islets (PMID: 18261235) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216323).

    • Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
      Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab226484？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226484 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab226484.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com