Anti-CD130 (gp130)抗体[B-S12] - BSA and Azide free (ab27359)
![Flow Cytometry - Anti-CD130 (gp130) antibody [B-S12] (ab27359)](https://www.abcam.cn/ps/products/27/ab27359/Images/ab27359-38124-anti-cd130-gp130-antibody-b-s12-flow-cytometry.gif "Flow Cytometry - Anti-CD130 (gp130) antibody [B-S12] (ab27359)")
Key features and details
- Mouse monoclonal [B-S12] to CD130 (gp130) - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD130 (gp130)抗体[B-S12] - BSA and Azide free
参阅全部 CD130 (gp130) 一抗 -
描述
小鼠单克隆抗体[B-S12] to CD130 (gp130) - BSA and Azide free -
宿主
Mouse -
特异性
Recognises the Gp130, common subunit for IL-6, IL-11, OSM, LIF, CNTF, CT-1 receptors, a 130-140 kDa protein.
Activates cells carrying gp130. Induces gp130 activation, Jak 1, Jak 2, Stat 1 and Stat 3 phosphorylation.
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经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to CD130 (gp130).
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阳性对照
- Eahy 926 cell line.
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常规说明
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Induces gp130 activation, Jak 1, Jak 2, Stat 1 and Stat 3 phosphorylation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
无载体
是 -
Concentration information loading... -
纯度
Tissue culture supernatant -
Primary antibody说明
Induces gp130 activation, Jak 1, Jak 2, Stat 1 and Stat 3 phosphorylation. -
克隆
单克隆 -
克隆编号
B-S12 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27359于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
组织特异性
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
序列相似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻译后修饰
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
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别名
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
图片
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Flow Cytometry - Anti-CD130 (gp130) antibody [B-S12] (ab27359)
A typical staining pattern with ab27359 using Eahy 926 cell line.
This image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab27359 被引用在 2 文献中.
- Huang H et al. lncRNA MALAT1 Promotes Renal Fibrosis in Diabetic Nephropathy by Targeting the miR-2355-3p/IL6ST Axis. Front Pharmacol 12:647650 (2021). PubMed: 33995063
- Fourcin M et al. gp130 transducing receptor cross-linking is sufficient to induce interleukin-6 type responses. J Biol Chem 271:11756-60 (1996). ELISA, Functional Studies . PubMed: 8662709