重组Anti-CD13抗体[EPR27472-288] (ab317440)
![Western blot - Anti-CD13 antibody [EPR27472-288] (ab317440)](https://www.abcam.cn/ps/products/317/ab317440/Images/ab317440-1-anti-cd13-antibody-epr27472-288-western-blot-j774a1-mouse-reticulum-cell-sarcoma-monocytemacrophage--whole-cell-lysate-20-ug.jpg "Western blot - Anti-CD13 antibody [EPR27472-288] (ab317440)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27472-288] to CD13
- Suitable for: Flow Cyt, WB, IP, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-CD13抗体[EPR27472-288]
参阅全部 CD13 一抗 -
描述
兔单克隆抗体[EPR27472-288] to CD13 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, WB, IP, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: J774A.1, Mouse kidney lysates. ICC: J774A.1 cells. Flow Cyt: Mouse spleen and J774A.1 cells. IP: J774A.1 cell.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27472-288 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317440于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/500.
|
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| WB |
1/1000. Predicted molecular weight: 109 kDa.
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| IP |
1/30.
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| ICC/IF |
1/50.
|
| 说明 |
|---|
|
Flow Cyt
1/500. |
|
WB
1/1000. Predicted molecular weight: 109 kDa. |
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IP
1/30. |
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ICC/IF
1/50. |
靶标
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功能
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. -
组织特异性
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients. -
序列相似性
Belongs to the peptidase M1 family. -
结构域
Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies). -
翻译后修饰
Sulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form. -
细胞定位
Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected. - Information by UniProt
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数据库链接
- Entrez Gene: 16790 Mouse
- SwissProt: P97449 Mouse
- Unigene: 4487 Mouse
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别名
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
see all
图片
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Western blot - Anti-CD13 antibody [EPR27472-288] (ab317440)All lanes : Anti-CD13 antibody [EPR27472-288] (ab317440) at 1/1000 dilution
Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate
Lane 2 : EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 109 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: EL4 (PMID: 8103749).
Low expression: heart.
The molecular weight observed is consistent with what has been described in the literature (PMID: 37942010, 8103749).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR27472-288] (ab317440)](https://www.abcam.cn/ps/products/317/ab317440/Images/ab317440-2-anti-cd13-antibody-epr27472-288-immunocytochemistry-j774a1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR27472-288] (ab317440)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CD13 with ab317440 at 1/50 (10.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing membranous staining in J774A.1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control: EL4 (PMID: 8103749).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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![Flow Cytometry - Anti-CD13 antibody [EPR27472-288] (ab317440)](https://www.abcam.cn/ps/products/317/ab317440/Images/ab317440-4-anti-cd13-antibody-epr27472-288-flow-cytometry-mouse-spleen-cell.jpg)
Flow Cytometry - Anti-CD13 antibody [EPR27472-288] (ab317440)Flow cytometric analysis of Mouse spleen cell cells labelling CD13 with ab317440 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti-CD11c conjugated to Brilliant Violet 421.
Gated on viable cells. -
![Flow Cytometry - Anti-CD13 antibody [EPR27472-288] (ab317440)](https://www.abcam.cn/ps/products/317/ab317440/Images/ab317440-5-anti-cd13-antibody-epr27472-288-flow-cytometry-mouse-spleen-cell1.jpg)
Flow Cytometry - Anti-CD13 antibody [EPR27472-288] (ab317440)Flow cytometric analysis of Mouse spleen cell cells labelling CD13 with ab317440 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti-CD3 conjugated to Alexa Fluor®647.
Gated on viable cells. -
![Flow Cytometry - Anti-CD13 antibody [EPR27472-288] (ab317440)](https://www.abcam.cn/ps/products/317/ab317440/Images/ab317440-6-anti-cd13-antibody-epr27472-288-flow-cytometry-j774a1.jpg)
Flow Cytometry - Anti-CD13 antibody [EPR27472-288] (ab317440)Flow cytometric analysis of J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) (Right) / EL4 (mouse lymphoma T lymphocyte) (Left) cells labelling CD13 with ab317440 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: EL4 (PMID: 8103749) -
![Immunoprecipitation - Anti-CD13 antibody [EPR27472-288] (ab317440)](https://www.abcam.cn/ps/products/317/ab317440/Images/ab317440-3-anti-cd13-antibody-epr27472-288-immunoprecipitation-j774a1.jpg)
Immunoprecipitation - Anti-CD13 antibody [EPR27472-288] (ab317440)CD13 was immunoprecipitated from 0.35 mg J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate with ab317440 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317440 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate
Lane 2: ab317440 IP in J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317440 in J774A.1 whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds.
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![Anti-CD13 antibody [EPR27472-288] (ab317440)](https://www.abcam.cn/ps/products/317/ab317440/Images/ab317440-7-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD13 antibody [EPR27472-288] (ab317440)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab317440 尚未被引用在任何文献中。