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Anti-CD11+CD18抗体[24] (ab13219)

Reviews (5)Q&A (3)References (24)

Key features and details

  • Mouse monoclonal [24] to CD11+CD18
  • Suitable for: IP, RIA, ICC/IF, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-CD11+CD18抗体[24]
    参阅全部 CD11+CD18 一抗

  • 描述

    小鼠单克隆抗体[24] to CD11+CD18

  • 宿主

    Mouse

  • 特异性

    This antibody specifically recognises the human CD11+CD18 activation epitope.

  • 经测试应用

    适用于: IP, RIA, ICC/IF, WB, Flow Cytmore details

  • 种属反应性

    与反应: Mouse, Rat, Dog, Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD11+CD18. Fibronectin-purified human monocytes.

  • 表位

    Recognises an epitope which is expressed by beta 2 (CD18) integrins in high affinity conformation.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab13219于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 129 kDa. PubMed: 22473604
Flow Cyt (5)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
IP
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 129 kDa. PubMed: 22473604
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

文献 (24)

发表研究结果有使用 ab13219？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab13219 被引用在 24 文献中.

  • Nandagopal S  et al. C3aR Signaling Inhibits NK-cell Infiltration into the Tumor Microenvironment in Mouse Models. Cancer Immunol Res 10:245-258 (2022). PubMed: 34819308
  • Rossatti P  et al. Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation. BMC Biol 20:189 (2022). PubMed: 36002835
  • Chen Y  et al. The Mechanism of Alisol B23 Acetate Inhibiting Lung Cancer: Targeted Regulation of CD11b/CD18 to Influence Macrophage Polarization. Drug Des Devel Ther 16:3677-3689 (2022). PubMed: 36277599
  • Bhagat S  et al. Key role of Extracellular RNA in hypoxic stress induced myocardial injury. PLoS One 16:e0260835 (2021). PubMed: 34882718
  • Cao JY  et al. Exosomal miR-125b-5p deriving from mesenchymal stem cells promotes tubular repair by suppression of p53 in ischemic acute kidney injury. Theranostics 11:5248-5266 (2021). PubMed: 33859745
View all Publications for this product

客户评价及客户问答

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1-8 of 8 Abreviews or Q&A

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 29 2012

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Dog Cell (blood leucocyte)
Specification
blood leucocyte
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
monocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 12 2012

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Poor
Abreviews
abreview image
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 04 2012

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: after Ficoll
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
monocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 18 2011

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Permeabilization
No
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 28 2011

Question



I need for a flow cytometry experiment using Anti-CD11+CD18 antibody [24] (ab13219) against LFA1 on THP1 cells. Please suggest Isotype controls.




Read More
Answer

The Anti-CD11+CD18 antibody [24] (ab13219) is a mouse IgG1 monoclonal antibody.
We have the following Isotype control that you can use for your flow cytometry experiment:
Mouse IgG1 [CT6] - Isotype control (ab81216), tested applications: Flow Cyr,IHC-P and WB,
https://www.abcam.com/Mouse-IgG1-CT6-Isotype-control-ab81216.html https://www.abcam.com/Mouse-IgG1-CT6-Isotype-control-ab81216.html.
This antibody has been referenced in 2 publications that you can visualize by clicking the following link:
https://www.abcam.com/mouse-igg1-ct6-isotype-control-ab81216-references.html
You can also find Isotype controls from our homepage by selecting the Control tab (top right) and selecting Isotype controls for the drop-down menu. You can use search filters on the left hand site to help find a suitable control for your experiments.

Read More

Question

Many thanks for your quick reply.

Can I just confirm that the antibody specifically recognizes active CD11a/CD18 (LFA-1) or does it all recognize active CD11b/c/d with CD18?

Thanks,

Read More
Answer

Thank you for your patience. My colleague is out of office this week.

I regret to inform you that we do not have any direct data that answers your question. However,we know the scientist described the antibody as being able to be used to detect the activation state of each integrin receptor which suggests that it does recognize CD11a, b, c and d.

Apologies! If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Question

Product code: 13219
Inquiry: I am looking for an antibody that will be able to distinguish between inactive and active LFA-1 (CD11a/CD18) expression. The data sheets suggest that the antibody is to an epitope on CD18 that is only exposed once it has dimerised with the alpha chain and the integrin has been activated? Is this specific for active LFA-1 or beta 2 integrins in general?

Read More
Answer

Thank you for contacting us.

The antibody was found to recognize a unique activation epitope of LFA-1, which is essentially absent on resting cells e.g. lymphocytes so this product may be used as a reporter of the activation state of CD11/CD18 receptors.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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