重组Anti-CD11b抗体[EPR1344] (ab133357)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1344] to CD11b
- Suitable for: mIHC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD11b抗体[EPR1344]
参阅全部 CD11b 一抗 -
描述
兔单克隆抗体[EPR1344] to CD11b -
宿主
Rabbit -
经测试应用
适用于: mIHC, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Pig, Rhesus monkey -
免疫原
Synthetic peptide within Human CD11b aa 1-100. The exact sequence is proprietary.
Database link: P11215 -
阳性对照
- WB: THP1 cell lysate treated with TPA, and TF1 cell lysate; Rat spleen lysate IHC-P: Human tonsil and spleen tissues; Rat cerebrum and bone marrow tissue; Mouse lung and colon tissue. mIHC: Mouse spleen tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1344 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- HRP Anti-CD11b antibody [EPR1344] (ab204472)
- Anti-CD11b antibody [EPR1344] - BSA and Azide free (ab209970)
- Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (ab216445)
- Alexa Fluor® 568 Anti-CD11b antibody [EPR1344] (ab313094)
- Alexa Fluor® 750 Anti-CD11b antibody [EPR1344] (ab321047)
- Biotin Anti-CD11b antibody [EPR1344] (ab322298)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133357于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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mIHC |
1/20000.
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WB | (4) |
1/1000. Predicted molecular weight: 127 kDa.
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IHC-P | (17) |
1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Please optimize IHC protocol when testing mouse and rat tissues. It is easy to show background staining in liver tissue. |
说明 |
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mIHC
1/20000. |
WB
1/1000. Predicted molecular weight: 127 kDa. |
IHC-P
1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Please optimize IHC protocol when testing mouse and rat tissues. It is easy to show background staining in liver tissue. |
靶标
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功能
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
组织特异性
Predominantly expressed in monocytes and granulocytes. -
疾病相关
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3684 Human
- Entrez Gene: 16409 Mouse
- Entrez Gene: 25021 Rat
- Entrez Gene: 714832 Rhesus monkey
- Omim: 120980 Human
- SwissProt: P11215 Human
- SwissProt: P05555 Mouse
- Unigene: 172631 Human
see all -
别名
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Immunohistochemical analysis of formalin fixed paraffin embedded human spleen labelling CD11b with ab133357 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab133357 Anti-CD11b antibody was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Immunohistochemical analysis of formalin fixed paraffin embedded human spleen labelling CD11b with ab133357 at a concentration of 0.32µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. ab133357Anti-CD11b antibody was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen labelling LY6C with ab317272 at 1/100 (B), CD11b with ab133357 at 1/20000 dilution (C) and CD19 with ab245235 at 1/1000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-LY6C (green; Opal™690), anti-CD11b (magenta; Opal™570) and anti-CD19 (yellow; Opal™520) on mouse spleen.
Panel B: anti-LY6C stained on monocytes/macrophages.
Panel C: anti-CD11b stained on monocytes/macrophages.
Panel D: anti-CD19 stained on B cells.
Co-staining of LY6C and CD11b can be observed.The section was incubated in three rounds of staining: in the order of ab317272, ab133357, and ab245235 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)Min, M et al PLoS One. 2017 Dec 22;12(12):e0190051. doi: 10.1371/journal.pone.0190051. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Rottlerin decreases the number of effector cells that mainly infiltrate the skin in IMQ-treated mice
Immunohistochemical detection of immune cell-related markers was performed on paraffin-embedded sections obtained from the back skin of IMQ-induced mice treated with vehicle or rottlerin.
Representatives IHC images of CD11b (B) on the skin of the vehicle or rottlerin-treated mice. Scale bar = 100μm.
Quantification analysis of IHC staining for CD11b(E) on the skin of the vehicle and rottlerin treated mice. Two independent researchers counted the number of positive staining cells were per high-power field (HPF). The data are representative of three experiments (n = 5 mice per group). ** P<0.01 vs. vehicle.
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All lanes : Anti-CD11b antibody [EPR1344] (ab133357) at 1/1000 dilution (purified)
Lane 1 : TF-1 cell lysate
Lane 2 : TPA treated THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)This image is courtesy of an abreview from Anugraha Rajagopalan
Formalin-fixed, 0.2% Triton-X permeabilized Mouse tissue sections (Eo771 breast cancer) was stained for CD11b using ab133357 (Green) at 1/1000 dilution in immunohistochemical analysis. The secondary antibody was a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) at 1/1000 dilution.
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Ab133357 staining CD11b in paraffin embedded Mouse lung tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/4000 dilution (0.031 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells of mouse lung.
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Different batches of ab133357 were tested on Rat spleen lysate at 0.1 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 170 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Ab133357 staining CD11b in paraffin embedded Mouse colon tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/4000 dilution (0.031 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells of mouse colon.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Ab133357 staining CD11b in paraffin embedded Rat cerebrum tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/4000 dilution (0.29 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on gliocytes of rat cerebrum [PMID: 20483006].
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Formaldehyde-fixed, paraffin-embedded rat bone marrow tissue stained for CD11b using ab133357 at 1/5000 in immunohistochemical analysis.
Heat mediated antigen retrieval with EDTA buffer pH 9 was performed before commencing with staining protocol. 1% casein was used as blocking agent.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
IHC image of CD11b staining in a formalin fixed, paraffin embedded human normal spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab133357 at 1/4000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Anti-CD11b antibody [EPR1344] (ab133357) at 1/1000 dilution + Rat spleen tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : purified at 1/10000 dilution
Lane 1 : RAW264.7 cell lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Immunohistochemical staining of paraffin embedded human spleen with purified ab133357 at a 1/4000 dilution. The secondary antibody used is a HRP goat anti-rabbit (ab97051). The sample is counterstained with hematoxylin.
Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Tissue Microarrays stained for "Anti-CD11b antibody [EPR1344]” using "ab133357" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab133357 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
Tissue Microarrays stained for "Anti-CD11b antibody [EPR1344]” using "ab133357" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab133357 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
数据表及文件
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SDS download
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Datasheet download
文献 (347)
ab133357 被引用在 347 文献中.
- Huang S et al. Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ. Methods Mol Biol 2713:99-115 (2024). PubMed: 37639117
- Zheng C et al. Tumor-specific cholinergic CD4+ T lymphocytes guide immunosurveillance of hepatocellular carcinoma. Nat Cancer 4:1437-1454 (2023). PubMed: 37640929
- Doha ZO et al. MYC Deregulation and PTEN Loss Model Tumor and Stromal Heterogeneity of Aggressive Triple-Negative Breast Cancer. Nat Commun 14:5665 (2023). PubMed: 37704631
- Tian L et al. C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages. iScience 26:107771 (2023). PubMed: 37731607
- Omatsu M et al. THBS1-producing tumor-infiltrating monocyte-like cells contribute to immunosuppression and metastasis in colorectal cancer. Nat Commun 14:5534 (2023). PubMed: 37749092