Anti-CD11b + CD11c抗体[OX42] - BSA and Azide free (ab238658)
Key features and details
- Mouse monoclonal [OX42] to CD11b + CD11c - BSA and Azide free
- Suitable for: ICC/IF, IHC-Fr
- Reacts with: Rat
- Isotype: IgG2a
概述
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产品名称
Anti-CD11b + CD11c抗体[OX42] - BSA and Azide free -
描述
小鼠单克隆抗体[OX42] to CD11b + CD11c - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: ICC/IF, IHC-Frmore details
不适用于: IHC-P -
种属反应性
与反应: Rat
不与反应: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: NR8383 cells, rat macrophages, IHC-Fr: frozen Rat lung tissue sections (10µm)
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常规说明
ab238658 is the carrier-free version of ab1211.
IHC protocol advice:
This antibody is not suitable for IHC on paraffin-embedded samples.
For best results in IHC on frozen tissue, the following may help detection:
1) Paraformaldehyde perfusion fixed samples have worked well for many customers.
2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861).
3) PFA-fixed samples will require cryoprotection by sucrose infiltration.
4) Use OCT (TissueTek) compound for embedding.
5) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min.
6) Do not heat the samples or sections.
7) During the staining procedure, do not allow the sections to dry out.Our Technical team (technical@abcam.com) will be happy to provide further information and advice.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
OX42 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab238658于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 - 10 µg/ml.
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IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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细胞定位
CD11b: Membrane. CD11c: Membrane. -
数据库链接
图片
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This data was developed using ab1211, the same antibody clone in a different buffer formulation.
Immunofluorescence staining of CD11b + CD11c staining in a section of 10% formalin fixed (10 mins, RT) frozen rat spleen.
Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab1211 at 1µg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1.5µg/ml) for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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NR8383 cells (rat macrophage cell line) stained for CD11b + CD11c (shown in green) using ab1211 in ICC/IF. The cells were fixed with 100% methanol for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% Tween-PBS for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1211 at 5µg/ml) overnight at +4°C. The secondary antibody was ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (shown in blue) at a concentration of 1.43 µM for 1 hour at room temperature.
This image was produced using the same antibody clone but in a different formulation; PBS, arginine and sodium azide (ab1211).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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