重组Anti-CD11a抗体[I21/7] - BSA and Azide free (ab272443)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [I21/7] to CD11a - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-CD11a抗体[I21/7] - BSA and Azide free
参阅全部 CD11a 一抗 -
描述
大鼠单克隆抗体[I21/7] to CD11a - BSA and Azide free -
宿主
Rat -
经测试应用
适用于: Flow Cytmore details
不适用于: IP -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Mouse splenocytes.
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常规说明
ab272443 is the carrier-free version of ab25383.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
克隆
单克隆 -
克隆编号
I21/7 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272443于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt
Use at an assay dependent concentration. |
靶标
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功能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. -
组织特异性
Leukocytes. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 16408 Mouse
- SwissProt: P24063 Mouse
- Unigene: 1618 Mouse
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别名
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
see all
图片
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Flow cytometric analysis of mouse splenocyte cells labelling CD11a with ab25383 at 0.963µg/ml (Right panel) compared with a rat monoclonal IgG isotype (Left panel). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab25383).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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