重组Anti-CD116抗体[EPR24554-113]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR24554-113] (AB280357)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD116 with ab280357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sporadic positive staining in mouse lung. The section was incubated with ab280357 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR24554-113] (AB280357)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD116 with ab280357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse spleen. The section was incubated with ab280357 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-CD116 antibody [EPR24554-113] (AB280357)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : Lane 1 : 80 seconds Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-CD116 antibody [EPR24554-113] (ab280357) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 60-70 kDa

false

• WB

Lab

Western blot - Anti-CD116 antibody [EPR24554-113] (AB280357)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Expression of GMRa is restrcted to cells of the myeloid, but not lymphoid (PMID 17694571).

No expression of GMRa is observed in mouse B cell lymphoma B lymphocyte WEHI-231.

The molecular weight observed is consistent with what has been described in the literature (PMID : 18955570).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CD116 antibody [EPR24554-113] (ab280357) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

J774A.1 (mouse reticum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

Lane 3:

WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 60-70 kDa

false