AB81456

重组Anti-CD105抗体[MJ7/18]

Anti-CD105 antibody [MJ7/18]

Recombinant
了解详情

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(1 Publication)

Rat Recombinant Monoclonal CD105 antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Mouse samples. Cited in 1 publication.

查看别名

CD105, Edg, Eng, Endoglin, Cell surface MJ7/18 antigen

2 Images

Flow Cytometry - Anti-CD105 antibody [MJ7/18] (AB81456)

Flow Cyt

Lab

Flow Cytometry - Anti-CD105 antibody [MJ7/18] (AB81456)

Flow cytometry overlay histogram showing left bEND.3 positive cells and right negative NIH3T3 cells stained with ab81456 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab81456) (1x10⁶ in 100 μl at 1 μg/ml) for 30 min on ice.

The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor^® 488, pre-adsorbed) (ab150165) was used at 1/2000 for 30 min on ice.

Isotype control antibody (black line) was Rat IgG2aκ (ab18450) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

Lab

Western blot - Anti-CD105 antibody [MJ7/18] (AB81456)

Lanes 1 - 3 : Merged signal (red and green). Green - ab81456 observed at 80 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

ab81456 was shown to react with CD105 in Western blot.

Membranes were blocked in 3% milk before incubation with ab81456 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 5 μg/ml and a 1 in 20000 dilution respectively.

Blots were incubated with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed (ab253031) and Goat anti-Rabbit IgG H&L (IRDye$®$ 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

Additional band(s) were observed at 45 kDa, these band(s) may represent alternative splice variants. This has not been investigated further.

Lane 1 : Mouse Lung tissue lysate (40 μg)
Lane 2 : Mouse Liver tissue lysate (40 μg)
Lane 3 : NIH/3T3 whole cell lysate (40 μg)

All lanes:

Western blot - Anti-CD105 antibody [MJ7/18] (ab81456) at 5 µg/mL

Lane 1:

Mouse Lung tissue lysate at 40 µg

Lane 2:

Mouse Liver tissue lysate at 40 µg

Lane 3:

NIH/3T3 whole cell lysate at 40 µg

Predicted band size: 70 kDa

Observed band size: 80 kDa

false

不同偶联物与剂型 (3)

578 PE

PE Anti-CD105 antibody [MJ7/18]
Biotin

Biotin Anti-CD105 antibody [MJ7/18]
Carrier free

Anti-CD105 antibody [MJ7/18] - BSA and Azide free

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

MJ7/18

亚型

IgG2a

不含载体蛋白

反应种属

Mouse

应用

IP, IHC-Fr, WB, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/10 - 1/20", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.1/10 - 1/20. Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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产品详情

This product was switched from a hybridoma to a recombinant production format on 27th October 2021.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Ion exchange chromatography

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 10625534). Required for normal structure and integrity of adult vasculature (By similarity). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (PubMed : 10625534). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (PubMed : 28530658). May play a role in the binding of endothelial cells to integrins. Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (By similarity).

See full target information Eng

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and 309:L942-58 PubMed26320158

2015

Characterization of the platelet-derived growth factor receptor-α-positive cell lineage during murine late lung development.

Applications

Unspecified application

Species

Unspecified reactive species

Aglaia Ntokou,Friederike Klein,Daria Dontireddy,Sven Becker,Saverio Bellusci,William D Richardson,Marten Szibor,Thomas Braun,Rory E Morty,Werner Seeger,Robert Voswinckel,Katrin Ahlbrecht

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