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Immunology Cell Type Markers CD Endothelial Cells
使用敲除细胞株进行验证

Anti-CD105抗体[MEM-226] (ab2529)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (17)

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Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
  • Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529)
  • Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529)
  • Western blot - Anti-CD105 antibody [MEM-226] (ab2529)

Key features and details

  • Mouse monoclonal [MEM-226] to CD105
  • Suitable for: ICC/IF, Sandwich ELISA, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-CD105抗体[MEM-226]
    参阅全部 CD105 一抗
  • 描述

    小鼠单克隆抗体[MEM-226] to CD105
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC/IF, Sandwich ELISA, Flow Cyt, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant full length protein (Human). Expressed in vaccinia virus containing CD105 cDNA.

  • 阳性对照

    • ICC/IF: HeLa cells. WB: Human colon tissue lysate. Flow Cyt: U937 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purified from TCS. Purity >95% by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    MEM-226
  • 同种型

    IgG1
  • 研究领域

    • Immunology
    • Cell Type Markers
    • CD
    • Endothelial Cells
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

相关产品

  • Alternative Versions

    • FITC Anti-CD105 antibody [MEM-226] (ab18278)
    • APC Anti-CD105 antibody [MEM-226], prediluted (ab60902)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human CD105 protein (ab54338)
  • Related Products

    • Recombinant human CD105 protein (ab54338)
  • sELISA pair antibody

    • Anti-CD105 antibody (ab21224)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2529于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 10 µg/ml.
Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to CD105 (ab21224). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to CD105 (ab21224) as Detection.
Flow Cyt
Use a concentration of 1 - 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration. Use under non reducing condition.
说明
ICC/IF
Use a concentration of 10 µg/ml.
Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to CD105 (ab21224). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to CD105 (ab21224) as Detection.
Flow Cyt
Use a concentration of 1 - 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration. Use under non reducing condition.

靶标

  • 功能

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • 组织特异性

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • 疾病相关

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P17813 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2022 Human
    • Omim: 131195 Human
    • SwissProt: P17813 Human
    • Unigene: 76753 Human
    • 别名

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    图片

    • Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
      Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
      All lanes : Anti-CD105 antibody [MEM-226] (ab2529) at 1/1000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : CD105 knockout HeLa whole cell lysate
      Lane 3 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.


      Lanes 1 - 3: Merged signal (red and green). Green - ab2529 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab2529 was shown to recognize ENG (Endoglin) in wild-type HeLa cells as signal was lost at the expected MW in ENG knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ENG knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab2529 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)
      Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)

      Overlay histogram showing U937 (Human histiocytic lymphoma cell line) cells stained with ab2529 (red line).

      The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2529, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in U937 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529)
      Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529)

      ICC/IF image of ab2529 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

      The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2529, 10 µg/ml) overnight at +4°C. The secondary antibody (green) was ab69879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    • Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529)
      Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529)
      Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml.
    • Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
      Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
      Anti-CD105 antibody [MEM-226] (ab2529) at 5 µg/ml + Human colon tissue lysate at 10 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Observed band size: 80 kDa why is the actual band size different from the predicted?
      Additional bands at: 45 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols
    • Protocol Booklet

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (17)

    发表研究结果有使用 ab2529?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2529 被引用在 17 文献中.

    • Liang L  et al. Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models. Stem Cell Res Ther 12:87 (2021). PubMed: 33494812
    • Zhang J  et al. Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats. Front Cell Dev Biol 9:629274 (2021). PubMed: 33644068
    • Bi R  et al. Identification of human temporomandibular joint fibrocartilage stem cells with distinct chondrogenic capacity. Osteoarthritis Cartilage 28:842-852 (2020). PubMed: 32147536
    • Taksima T  et al. Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer's Disease. Mar Drugs 17:N/A (2019). PubMed: 31690015
    • Chen Y  et al. Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds. J Cell Mol Med 23:5956-5969 (2019). PubMed: 31293077
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    We had recently buy a GPCR EX33 antibody (ab12529) from your company. I tested for FACS (fluorochrme detection with Alexa 488 coupled to anti-rabbit IGg (H+L) secondary antibody). My control was ok, but all cells were detected for GPR EX33 highly, but this must to be detectable highly just after LPS stimulation, and all cells traited with saline was highly expressed for this GPCR. In quantitative PCR, the transcript is very low detectable for the vehicle cells traited with saline and very high with LPS. Also, I was processed to blockade step before primary antibody incubation, with goat serum. Do you have other suggest for the optimal blockade step, because many GPCR are similarity between their recognizing domains, and also should I use a human anti-FCy? Maybe that this antibody is not very specific also (purification) ? I will wait your help,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 14 2005

    Answer

    I'm sorry to hear you are having problems with ab12529; this antibody has not been tested for FACS so it is very difficult to know if the problem can be solved with protocol problems or is due to the antibody not working well in FACS. As the antibody has been tested in IHC-P it means the antibody has the ability to recognise the native form of the protein, however this may require fixation and other protocol changes to get it working. The antibody has been tested in human brain tissue, so I would recommend trying this positive control if possible in IHC-P. The antibody has been purified on a peptide immunogen affinity column so it is purified and recognizes only GPCR EX33. You may be able to decrease the non specific staining with decreasing the antibody concentration and incubation conditions and changing the buffer. Could you please provide us your specific protocol details and we will look into this problem in more detail and hopefully will be able to suggest some tips? I enclose below a link to a protocol questionnaire to help you put your protocol information together easily, we look forward to hearing from you to help you more, https://www.abcam.com/index.html?section=facs&pageconfig=technical&intAbID=12529&mode=questionaire

    Read More

    Abcam Scientific Support

    回复于 Dec 16 2005

    Question

    I am looking for an antibody or antiserum against rat CD105. Thank you very much for your help to find this antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2003

    Answer

    Thank you for your enquiry. Unfortunately, we currently do not have an antibody or antiserum against rat CD105. To see if another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249). If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Nov 21 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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