重组Anti-CD105抗体[EPR10145-12] (ab169545)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10145-12] to CD105
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD105抗体[EPR10145-12]
参阅全部 CD105 一抗 -
描述
兔单克隆抗体[EPR10145-12] to CD105 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CD105 aa 1-200.
Database link: P17813 -
阳性对照
- WB: HeLa, ECV-304 and HUVEC cell lysates; Human tonsil tissue lysate; Immunoprecipitation pellet from ECV-304 cell lysate. IHC-P: Human glioma, clear cell carcinoma, tonsil and kidney tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10145-12 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab169545于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 70 kDa.
For unpurified use at 1/50. |
IHC-P | (2) |
1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/30. |
说明 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 70 kDa. For unpurified use at 1/50. |
IHC-P
1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30. |
靶标
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功能
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
组织特异性
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
疾病相关
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
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别名
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
图片
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All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ENG knockout HeLa cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab169545 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.
ab169545 Anti-CD105 antibody [EPR10145-12] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab169545 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : CD105 knockout HeLa whole cell lysate
Lane 3 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 70 kDaLanes 1 - 3: Merged signal (red and green). Green - ab169545 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab169545 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab169545 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with unpurified ab169545 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
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All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution (unpurified)
Lane 1 : ECV-304 cell lysate
Lane 2 : Human tonsil cell lysate
Lane 3 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 70 kDa -
Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution (unpurified) + ECV-304 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution (purified) + ECV-304 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution (unpurified) + immunoprecipitation pellet from ECV-304 cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 70 kDa -
Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution (unpurified) + HUVEC cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution (purified) + HUVEC cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with purified ab169545 at 1/900. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human clear cell carcinoma tissue labelling CD105 with unpurified ab169545 at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling CD105 with unpurified ab169545 at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD105 with unpurified ab169545 at 1/250.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab169545 被引用在 16 文献中.
- Fattahi F et al. Expressions of TWIST1 and CD105 markers in colorectal cancer patients and their association with metastatic potential and prognosis. Diagn Pathol 16:26 (2021). PubMed: 33752711
- Huang J et al. Myocardial infarction cardiomyocytes-derived exosomal miR-328-3p promote apoptosis via Caspase signaling. Am J Transl Res 13:2365-2378 (2021). PubMed: 34017395
- Sharma R et al. Determinants of resistance to VEGF-TKI and immune checkpoint inhibitors in metastatic renal cell carcinoma. J Exp Clin Cancer Res 40:186 (2021). PubMed: 34099013
- Sui T et al. Gambogic amide inhibits angiogenesis by suppressing VEGF/VEGFR2 in endothelial cells in a TrkA-independent manner. Pharm Biol 59:1566-1575 (2021). PubMed: 34767490
- Chun J et al. Osteogenic differentiation and inflammatory response of recombinant human bone morphogenetic protein-2 in human maxillary sinus membrane-derived cells. Exp Ther Med 20:81 (2020). PubMed: 32968438