Anti-CD105抗体(ab21224)

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab21224 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. After looking at the protocols you used, I am surprised that even using 50ug of protein did not manage to detect any bands. However, it is very hard for me to determine the cause without more details of your WB protocol. If you would be so kind to clarify the following items, it will certainly help me better understand the root of the cause and as a result, I will be able to tag this product as faulty. - did you used a lysis buffer? Was it as strong as RIPA buffer? Please try RIPA buffer as it is the strongest and recommend for extracting membrane bound proteins. - could the no signal you are experiencing be due to poor transfer of protein to membrane? Did you check the transfer efficiency? This can be simply done with a reversible stain such as Ponceau S. - At Abcam, almost all of our products has been tested with 5% BSA. Sometimes, a different blocking buffer might cause cross reaction between the blocking agent and antibodies, therefore causing no bands to appear. I would recommend you try 5% BSA, if you have not already done so. - can you confirm that the second antibody you used also managed to produce good results with other primary antibodies? What was the dilution you used? - was the incubation of the primary antibody done overnight at 4oC? - what type of membrane did you used? - can you confirm the washing buffer used? Was the washing done 15min x 3 times? This is because over washing will eventually cause you to lose signals. I am sorry for the string of questions but I hope the above recommendations may already help you. If you have already tried the above suggestions and still experience problems, please do not hesitate to contact me with details of your order (contact information, shipping address/purchasing agent, etc.). The purchase number you gave was not found in our system. It should be a 6 digit number. Also, please advice me on how you would like to proceed with your enquiry, so that I can immediately arrange for a replacement or refund for you.