Anti-CD105抗体[105C02] (ab44967)
Key features and details
- Mouse monoclonal [105C02] to CD105
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD105抗体[105C02]
参阅全部 CD105 一抗 -
描述
小鼠单克隆抗体[105C02] to CD105 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Full length protein corresponding to CD105. Purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
105C02 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab44967于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 95 kDa.
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说明 |
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WB
1/1000. Detects a band of approximately 95 kDa. |
靶标
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功能
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
组织特异性
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
疾病相关
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
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别名
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
图片
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All lanes : Anti-CD105 antibody [105C02] (ab44967) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : CD105 knockout HeLa whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Observed band size: 95 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab44967 observed at 95 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab44967 was shown to specifically react with CD105 in wild-type HeLa cells as signal was lost in CD105 knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab44967 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab44967 被引用在 12 文献中.
- Hopf A et al. Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering. Bioengineering (Basel) 9:N/A (2022). PubMed: 36134958
- Wartalski K et al. Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro. Histochem Cell Biol 156:349-362 (2021). PubMed: 34269874
- Dinh PC et al. Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis. Nat Commun 11:1064 (2020). PubMed: 32111836
- Pan C et al. Pretreatment with human urine-derived stem cells protects neurological function in rats following cardiopulmonary resuscitation after cardiac arrest. Exp Ther Med 20:112 (2020). PubMed: 32989390
- Bordin A et al. Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells. Stem Cells Int 2019:1516746 (2019). PubMed: 30755774