为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
重组RabMAb

重组Anti-CD103抗体[EPR4166(2)] (ab129202)

  • Datasheet
  • SDS
Reviews (8)Q&A (3)References (40)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • OI-RD Scanning - Anti-CD103 antibody [EPR4166(2)] (ab129202)
  • Anti-CD103 antibody [EPR4166(2)] (ab129202)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4166(2)] to CD103
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

你可能会感兴趣

一抗
Product image
Anti-ICOS antibody [SP98] (ab105227)
蛋白
Product image
Recombinant Human CD103 protein (ab114703)
一抗
Product image
Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free (ab209889)

查看更多相关产品

概述

  • 产品名称

    Anti-CD103抗体[EPR4166(2)]
    参阅全部 CD103 一抗
  • 描述

    兔单克隆抗体[EPR4166(2)] to CD103
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide within Human CD103 aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: P38570

  • 阳性对照

    • IHC-P: Human colon, tonsil, stomach and ileum tissues.
  • 常规说明

    Clone EPR4166(2) is different from clone EPR4166.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD)

    KD = 1.62 x 10 -11 M
    Learn more about KD
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR4166(2)
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

相关产品

  • Alternative Versions

    • Anti-CD103 antibody [EPR4166(2)] - Low endotoxin, Azide free (ab221210)
    • Alexa Fluor® 647 Anti-CD103 antibody [EPR4166(2)] (ab225153)
    • Anti-CD103 antibody [EPR4166(2)] - BSA and Azide free (ab271889)
    • Alexa Fluor® 568 Anti-CD103 antibody [EPR4166(2)] (ab313073)
    • Alexa Fluor® 750 Anti-CD103 antibody [EPR4166(2)] (ab321038)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD103 protein (ab114703)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab129202于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (5)
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

说明
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

靶标

  • 功能

    Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
  • 组织特异性

    Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
  • 序列相似性

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • 结构域

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P38570 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3682 Human
    • Omim: 604682 Human
    • SwissProt: P38570 Human
    • Unigene: 513867 Human
    • 别名

      • A530055J10 antibody
      • alpha-E1 antibody
      • alpha-M290 antibody
      • aM290 antibody
      • Antigen CD103 antibody
      • CD 103 antibody
      • CD103 antibody
      • CD103 antigen antibody
      • HML 1 antigen antibody
      • HML-1 antigen antibody
      • HML1 antigen antibody
      • Human mucosal lymphocyte antigen 1 antibody
      • Human mucosal lymphocyte antigen 1, alpha subunit antibody
      • Huminae antibody
      • Integrin alpha E antibody
      • Integrin alpha E heavy chain antibody
      • Integrin alpha E light chain antibody
      • Integrin alpha E, epithelial-associated antibody
      • Integrin alpha E1 antibody
      • Integrin alpha IEL antibody
      • Integrin alpha M290 antibody
      • Integrin alpha-E heavy chain antibody
      • Integrin alpha-IEL antibody
      • Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody
      • Integrin, alpha E antibody
      • ITAE_HUMAN antibody
      • Itgae antibody
      • MGC141996 antibody
      • MGC183601 antibody
      • Mucosal lymphocyte 1 antigen antibody
      • Mucosal lymphocyte antigen 1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)This image is courtesy of an Abreview submitted by Joshua Dodge
      Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde-fixed human Tonsil tissue. Stained with ab129202 at 1/1000 dilution.
      Secondary antibody used was Rabbit IgG VisUCyte HRP Polymer Antibody. Blocking was done with Bloxall Endogenous Peroxidase and Alkaline Phosphatase Blocking for 10 minutes at 25°C. The sample was incubated with the primary antibody and 1% BSA in PBS Buffer for 24 hours at 4°C. Antigen retrieval method was heat mediated, Citrate pH6.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling CD103 with purified ab129202 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

      Negative control using PBS instead of primary antibody (inset).

      Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling CD103 with unpurified ab129202 at a dilution of 1/500.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

      Anti-CD103/Integrin alpha E antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD103/Integrin alpha E with ab129202 at a dilution of 1:1000. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
      This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labeling CD103 with unpurified ab129202.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human ileum tissue labeling CD103 with unpurified ab129202.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

      Unpurified ab129202 showing positive staining or CD103 in normal stomach tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

      Unpurified ab129202 showing negative staining in colonic adenocarcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

      Unpurified ab129202 showing negative staining in normal uterus tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      OI-RD Scanning - Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CD103 antibody [EPR4166(2)] (ab129202)
      Anti-CD103 antibody [EPR4166(2)] (ab129202)

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (40)

    发表研究结果有使用 ab129202?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab129202 被引用在 40 文献中.

    • Hyung J  et al. Clinical Outcomes of Small Cell Carcinoma of the Genitourinary Tract and the Prognostic Significance of the Tumor Immune Microenvironment. Cancer Res Treat 56:624-633 (2024). PubMed: 38037320
    • Eschweiler S  et al. JAML immunotherapy targets recently activated tumor-infiltrating CD8+ T cells. Cell Rep 42:112040 (2023). PubMed: 36701231
    • Miyagawa C  et al. Histopathological subtyping of high-grade serous ovarian cancer using whole slide imaging. J Gynecol Oncol 34:e47 (2023). PubMed: 36807749
    • Chen L  et al. Prognostic values of tissue-resident CD8+T cells in human hepatocellular carcinoma and intrahepatic cholangiocarcinoma. World J Surg Oncol 21:124 (2023). PubMed: 37024870
    • Kim JH  et al. Clinical implications of the tumor microenvironment using multiplexed immunohistochemistry in patients with advanced or metastatic renal cell carcinoma treated with nivolumab plus ipilimumab. Front Oncol 12:969569 (2022). PubMed: 36237314
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 11 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD103 antibody [EPR4166(2)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (small intestine)
    Permeabilization
    No
    Specification
    small intestine
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. 勝啓 平田

    Verified customer

    提交于 Oct 14 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD103 antibody [EPR4166(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: BOND ER2
    Permeabilization
    No
    Specification
    intestine
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. 勝啓 平田

    Verified customer

    提交于 May 12 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-CD103 antibody [EPR4166(2)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Duodenum)
    Permeabilization
    No
    Specification
    Duodenum
    Blocking step
    V-blocker as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Li Li

    Verified customer

    提交于 Aug 01 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD103 antibody [EPR4166(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    Bloxall Endogenous Peroxidase and Alkaline Phosphatase Blocking as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Joshua Dodge

    Verified customer

    提交于 Jan 22 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Integrin alpha E antibody [EPR4166(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Ovarian Tumor)
    Permeabilization
    Yes - PBS + 0.3% Triton X-100
    Specification
    Ovarian Tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 26 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha E antibody [EPR4166(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (doudenum celiac disease with intraepithelial lymph)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20 min
    Permeabilization
    No
    Specification
    doudenum celiac disease with intraepithelial lymph
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 29 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha E antibody [EPR4166(2)]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mammary tumor (MMATV-PyMT))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 5 min high pressure
    Permeabilization
    Yes - DAKO TRS
    Specification
    Mammary tumor (MMATV-PyMT)
    Blocking step
    5% goat serum +3% BSA in IF buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 14 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha E antibody [EPR4166(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Tonsil-cynomolgus monkey)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0
    Permeabilization
    No
    Specification
    Tonsil-cynomolgus monkey
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Jing Ma

    Verified customer

    提交于 Jul 30 2012

    Question

    Hi Technical,



    We have a customer who is interested in purchasing a few vials of this antibody is wondering what the concentration of the lot you have in stock is.



    Hoping to hear back from you soon.





    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 13 2012

    Answer

    Thank you for your inquiry.

    We currently have two vials in stock. They both have a concentration of 0.948 mg/ml.

    Icannot confirmthe concentration of future lots.

    I also would like to comment on the concentration:

    The IgG produced by rabbits have greater affinity and greater sensitivity than mouse IgG (about 100-1000 fold) and at the same time the total amount of IgG produced by the rabbit hybridomas is generally lower than in mouse hybridomas.

    Many 'veteran' antibody users are generally familiar with the IgG amount provided from mouse antibodies and can judge how many reactions they may get or need to use based on concentration. Unfortunately, this experience is not transferable to rabbit monoclonal antibodies because the concentration scales are generally very different.

    Since the specificity and sensitivity is much higher with rabbit monoclonal antibodies, less IgG is required per reaction.

    I would like to reassure you that while the amount of IgG may not be comparable to mouse monoclonal antibodies, customers are receiving a comparable number of reactions/tests/blots/stainings per tube.

    I hope this information is helpful. Please do not hesitate to contact me with any further questions in this matter.

    Read More

    Abcam Scientific Support

    回复于 Aug 13 2012

    Question

    Testing discount code to test ab129202 in Cyno Monkey IHC-P samples.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 05 2012

    Answer

    DISCOUNT CODE: *
    Expiration date:*

    I am very pleased to hear you would like to accept our offer and test ab129202 in Cyno Monkey. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Cyno Monkey and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Jul 05 2012

    1-10 of 11 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2025 艾博抗(上海)贸易有限公司版权所有