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AB243934

重组Anti-CD10抗体[SP179] - BSA and Azide free

Anti-CD10 antibody [SP179] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD10 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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CD10, EPN, MME, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Formalin-fixed, paraffin-embedded human liver tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Intracellular flow cytometric analysis ofRamos (human Burkitt's lymphoma cell line) cell line labeling CD10 with ab227659 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Formalin-fixed, paraffin-embedded human breast tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Formalin-fixed, paraffin-embedded human prostate tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Formalin-fixed, paraffin-embedded human kidney tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/20 dilution (7.05μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Formalin-fixed, paraffin-embedded human placenta tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Intracellular Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/200 dilution (0.705μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659).

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Intracellular Flow Cytometry analysis of 2.4G2 (Rat B cell lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/200 dilution (0.705μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659)

不同偶联物与剂型 (10)

  • Unconjugated

    Anti-CD10 antibody [SP179] - N-terminal

  • 660 APC

    APC Anti-CD10 antibody [SP179] (N-terminal)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD10 antibody [SP179] - N-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD10 antibody [SP179] - N-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD10 antibody [SP179] - N-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD10 antibody [SP179] - N-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD10 antibody [SP179] - N-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CD10 antibody [SP179] - N-terminal

  • HRP

    HRP Anti-CD10 antibody [SP179] (N-terminal)

  • 578 PE

    PE Anti-CD10 antibody [SP179] (N-terminal)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

SP179

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" } } }

产品详情

ab243934 is the carrier-free version of ab227659.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
纯化说明
Purified from TCS by protein A/G.
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.
Biological function summary

CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.

Pathways

Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.

CD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed : 15283675, PubMed : 6208535, PubMed : 6349683, PubMed : 8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed : 17101991, PubMed : 6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed : 6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed : 15283675, PubMed : 6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed : 16254193, PubMed : 2531377, PubMed : 2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed : 20876573).
See full target information MME

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