重组Anti-CD10抗体[SP179] - BSA and Azide free
Anti-CD10 antibody [SP179] - BSA and Azide free
- RabMAb
- Recombinant
- 了解详情
Be the first to review this product! Submit a review
|
(0 Publication)
Rabbit Recombinant Monoclonal CD10 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
查看别名
CD10, EPN, MME, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Formalin-fixed, paraffin-embedded human liver tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Intracellular flow cytometric analysis ofRamos (human Burkitt's lymphoma cell line) cell line labeling CD10 with ab227659 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Formalin-fixed, paraffin-embedded human breast tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Formalin-fixed, paraffin-embedded human prostate tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Formalin-fixed, paraffin-embedded human kidney tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/20 dilution (7.05μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Formalin-fixed, paraffin-embedded human placenta tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227659).
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Intracellular Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/200 dilution (0.705μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659).
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Intracellular Flow Cytometry analysis of 2.4G2 (Rat B cell lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/200 dilution (0.705μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - BSA and Azide free (AB243934)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227659)
不同偶联物与剂型 (10)
-
Anti-CD10 antibody [SP179] - N-terminal
-
660 APC
APC Anti-CD10 antibody [SP179] (N-terminal)
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-CD10 antibody [SP179] - N-terminal
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-CD10 antibody [SP179] - N-terminal
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-CD10 antibody [SP179] - N-terminal
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-CD10 antibody [SP179] - N-terminal
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-CD10 antibody [SP179] - N-terminal
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-CD10 antibody [SP179] - N-terminal
-
HRP Anti-CD10 antibody [SP179] (N-terminal)
-
578 PE
PE Anti-CD10 antibody [SP179] (N-terminal)
反应性数据
产品详情
ab243934 is the carrier-free version of ab227659.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能和储存信息
形式
纯化工艺
纯化说明
存储溶液
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.
Pathways
Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com