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Immunology Adaptive Immunity B Cells CD

Anti-CD10抗体[56C6] (ab951)

  • Datasheet
  • SDS
Reviews (5)Q&A (10)References (34)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)

Key features and details

  • Mouse monoclonal [56C6] to CD10
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-CD10抗体[56C6]
    参阅全部 CD10 一抗
  • 描述

    小鼠单克隆抗体[56C6] to CD10
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment corresponding to CD10. Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein

  • 阳性对照

    • IHC-P: MDA-MB-231 human breast cancer cells grown in mouse. Renal cell carcinoma tissue.
  • 常规说明

    We have mixed customer feedback about the mouse specificity, which is why we no longer guarantee this species. Contact scientific support team for any inquiry.

    This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituents: PBS, Carrier protein

    Renoir Red Diluent
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    56C6
  • 同种型

    IgG1
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor immunology
    • CD markers

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human CD10 protein (ab157051)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab951于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (3)
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-).
说明
IHC-P
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-).

靶标

  • 功能

    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • 序列相似性

    Belongs to the peptidase M13 family.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P08473 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4311 Human
    • Entrez Gene: 17380 Mouse
    • Omim: 120520 Human
    • SwissProt: P08473 Human
    • SwissProt: Q61391 Mouse
    • Unigene: 307734 Human
    • Unigene: 296022 Mouse
    • 别名

      • Atriopeptidase antibody
      • CALLA antibody
      • CD10 antibody
      • CD10 antigen antibody
      • Common acute lymphocytic leukemia antigen antibody
      • DKFZp686O16152 antibody
      • EC 3.4.24.11 antibody
      • Enkephalinase antibody
      • EPN antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
      • Membrane metallo endopeptidase antibody
      • Membrane metallo endopeptidase variant 1 antibody
      • Membrane metallo endopeptidase variant 2 antibody
      • Membrane metalloendopeptidase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
      • Membrane metalloendopeptidase variant 1 antibody
      • Membrane metalloendopeptidase variant 2 antibody
      • MGC126681 antibody
      • MGC126707 antibody
      • MME antibody
      • NEP antibody
      • NEP_HUMAN antibody
      • Neprilysin antibody
      • neprilysin-390 antibody
      • neprilysin-411 antibody
      • Neutral endopeptidase 24.11 antibody
      • Neutral endopeptidase antibody
      • Neutral endopeptidase, membrane-associated antibody
      • SFE antibody
      • Skin fibroblast elastase antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)

      Paraffin embedded human renal cell carcinoma tissue stained for CD10 with ab951 (1/25 dilution) in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)This image is courtesy of an anonymous Abreview.

      ab951 at 1/20 dilution staining CD10 in MDA-MB-231 human breast cancer cells grown in mouse by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C and then incubated with ab951 for 17 hours at 4°C. A rabbit polyclonal to mouse Ig, diluted 1/200 was used as the secondary antibody.

      See Abreview

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (34)

    发表研究结果有使用 ab951?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab951 被引用在 34 文献中.

    • Lackie RE  et al. Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-ß deposition in a mouse model of Alzheimer's disease. Acta Neuropathol Commun 8:143 (2020). PubMed: 32825842
    • Kobayashi T  et al. Retrograde transport of masseter muscle-derived neprilysin to hippocampus. Neurosci Lett 698:180-185 (2019). PubMed: 30639512
    • Gill M  et al. Placental Syncytiotrophoblast-Derived Extracellular Vesicles Carry Active NEP (Neprilysin) and Are Increased in Preeclampsia. Hypertension 73:1112-1119 (2019). PubMed: 30929513
    • Hu T  et al. Neuroprotection induced by Navß2-knockdown in APP/PS1 transgenic neurons is associated with NEP regulation. Mol Med Rep 20:2002-2011 (2019). PubMed: 31257483
    • Kim KS  et al. Oral Nanoparticles Exhibit Specific High-Efficiency Intestinal Uptake and Lymphatic Transport. ACS Nano 12:8893-8900 (2018). PubMed: 30088412
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 15 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD10 antibody [56C6]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Uterus)
    Permeabilization
    Yes - 0.5% Triton/PBS
    Specification
    Uterus
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 29 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD10 antibody [56C6]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (DLCBL cell line WSUDLCL2 xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
    Permeabilization
    No
    Specification
    DLCBL cell line WSUDLCL2 xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 16 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD10 antibody [56C6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mammary gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM pH=6.0
    Permeabilization
    Yes - PBS with 3% Triton
    Specification
    mammary gland
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 04 2015

    Question

    We have purchased Mouse monoclonal [56C6] to CD10 ab recently and could you please provide the concentration?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 15 2013

    Answer


    The IgG concentration of ab951 is 7.2µg/mL.

    Read More

    Abcam Scientific Support

    回复于 Mar 15 2013

    Question

    the ordered was placed on xxxx, the PO number is xxx. We would like another Ab951 sample (but of different lot , last lot is  xxxx. Below is the shipping address:  

    Read More

    Abcam community

    Verified customer

    Asked on Sep 27 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Sep 27 2012

    Question

    I followed exactly the protocol in the paper by Devi L et al you cited, unfortunately, I still did not see any ~100kd band ( see the attached jpg, the whole brain homogenates sample lane),  I also adopted another protocol to fractionate the mouse brain into cytosol and membrane fractions ( FEBS J, 2010, 277(14), 3051-3067) , neither did I see any ~100kd band from these samples. This is a really frustrating results. I would like to get either a refund or a free sample from Abcam. Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2012

    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    I am sorry that these protocol details did not yield the expected results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    I apologize for the inconvenience. If you have contacted us within six months of purchase I am pleased to offer you a free of charge replacement, or refund in compensation. In order to process this could you please provide me with the Abcam order confirmation number or the PO number and date of purchase?

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    回复于 Sep 26 2012

    Question

    Inquiry: Dear Madam and Sir, We bought Mouse anti-CD10 (56C6) N:ab951, lot Number: GR38260-2. I am wondering if you could tell me the Ab concentration of this product exactly. Thank you very much and best regards

    Read More

    Abcam community

    Verified customer

    Asked on Sep 19 2012

    Answer

    Thank you for contacting us and your interest in our products.

    The concentration of the Anti-CD10 antibody [56C6] (ab951), lot number GR38260-2 is 7.2µg/mL.

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    回复于 Sep 19 2012

    Question

    We have purchased two tubes of mouse CD10 antibody (56C6, catalogue ab951). Both of them give me expected band right below 100KD for NB7 cell lysate ( human neuroblastoma cell line known to have high CD10 expression) and 293T cells transfected with pLV-hNEP (expressing human CD10).  However, i could not get any correct CD1O band from my mouse brain lysates, which was very frustrating, we are in urgent need of this piece of in vivo data to complete the manuscript. I included a picture of the blot, I am wondering the the antibody itself is not good at detecting mouse CD10, since so far I only can get band of human CD10, not from mouse brain ( I also couldn’t detect cd10 with 200ug mouse brain lysate in another blot), not from mouse primary neuron culture either. Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on Aug 31 2012

    Answer

    Thank you for contacting us.

    I am sorry that this product has not been performing as it should in mouse.This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue.

    If you could provide me with the details of your protocol, I would be very happy to review those and see if there are any suggestions I acn make which will help.

    I have also found the protocol used byDevi L et al.Mechanisms underlying insulin deficiency-induced acceleration of ß-amyloidosis in a mouse model of Alzheimer's disease.PLoS One7:e32792 (2012).http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=22403710&dopt=Abstract

    They were able to use this product successfully in mouse tissue and their protocol information may help. I have pasted this below:

    Hemibrain samples were taken from the mice under deep isoflurane anesthesia and were snap-frozen for biochemical assays. For western blot analysis, each sample was homogenized in 5 volumes of modified RIPA buffer containing 150 mM NaCl, 50 mM Tris HCl (pH 8.0), 1 mM EDTA, 1% IGEPAL, 0.5% sodium deoxycholate, 0.1% SDS and protease/phosphatase inhibitor cocktail (Calbiochem, La Jolla, CA, USA), and centrifuged at 10,000 g for 10 min to remove any insoluble material. Protein concentrations were determined by a BCA protein assay kit (Pierce, Rockford, IL, USA), and 20–50 µg of protein was run on 4–12% NuPAGE gels (Invitrogen, Carlsbad, CA, USA) and transferred to nitrocellulose membranes. After blocking, membranes were probed with anti-neprilysin 1:2,000, (ab951, Abcam, Cambridge, MA, USA). They were then incubated with horseradish peroxidase-conjugated secondary IgG. Immunoblot signals were visualized by an ECL chemiluminescence substrate reagent kit and were quantified by densitometric scanning and image analysis using Quantity One software.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Sep 01 2012

    Question

    Product code: 951
    Lot number: GR79429-1
    Inquiry: How should the Renoir Red solution be used in conjunction with the CD10 antibody? Also, no concentration was listed on the tube or the product sheet, although it was specified that the antibody should be used at 5 ug/ml. What is the concentration of the 250 ul of CD10 antibody (ab951)?

    Read More

    Abcam community

    Verified customer

    Asked on May 08 2012

    Answer

    Thank you for contacting us.

    The IgG concentration for lot GR79429-1 is 7.2µg/mL. For IHC, the antibody should be used at a concentration of 1/20 - 1/50. The antibody was validated for IHC with a sensitive polymer detection system, so if you are using a different kind of detection, for instance an ABC kit, or a conjugated secondary without any ABC or polymer amplification, we recommend the lower dilution of 1/20. A bottle of the diluent Renoir Red is included as the optimal diluent. Just substitute it in place of your standard buffer diluent.

    I hope this helps. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 May 08 2012

    Question

    LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands. The expected molecular weight is 85 kDa, whereas I got a major slower band at about 100 kDa (which I believe is the correct band) and a faster, minor band at 75 kDa. Does the protein degrades/alterates upon boiling? Samples were, as usual, boiled for 3' before loading. SAMPLE Mouse brain extract. PRIMARY ANTIBODY AbCam ab951 DETECTION METHOD ECL and X-ray film ANTIBODY STORAGE CONDITIONS Antibody was supplied already dissolved and was stored at 4°C, as indicated. SAMPLE PREPARATION Fractionated extraction using: Fraction 1) Tris-HCl buffer, aprotinin, leupeptin, NP40, SDS, PMSF, Na3VO4, EDTA. Fraction 2) TNT buffer Fraction 3) Same as buffer 1, but with the addition of deoxycolate and EGTA in place of EDTA. AMOUNT OF PROTEIN LOADED 10 micrograms. ELECTROPHORESIS/GEL CONDITIONS Reducing 4-12% Bis-Tris gel, XT-MES BioRad buffer. TRANSFER AND BLOCKING CONDITIONS Used the TurboBlot transfer system by BioRad on nitrocellulose membrane. Blocking 4% non-fat dry milk in 0.2% Tween-TBS for 2h at R/T. SECONDARY ANTIBODY Jackson HRP-conjugated goat anti-mouse HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

    Read More

    Abcam community

    Verified customer

    Asked on Feb 23 2012

    Answer

    Thank you for contacting Abcam.

    I think you are correct that the band that you are seeing at 100kDa is correct as based on the Swissprot sequence http://www.uniprot.org/uniprot/Q61391, there are q few glycosylation sites on this protein that would account for the increase in the predicted molecular weight. The 75kDa mat be a non-specific band in which case changing your blocking reagent to 5%BSA may help to reduce that signal. Otherwise if it is a breakdown product then you could increase the amount of protease inhibitors that you are using.

    The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    回复于 Feb 23 2012

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