Anti-CCT3抗体(ab225878)
Key features and details
- Rabbit polyclonal to CCT3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CCT3抗体
参阅全部 CCT3 一抗 -
描述
兔多克隆抗体to CCT3 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Orangutan
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免疫原
Synthetic peptide within Human CCT3 aa 300-350. The exact sequence is proprietary. (NP_005989.3).
Database link: P49368 -
阳性对照
- IHC-P: Human lung carcinoma tissue; Mouse teratoma tissue. WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab225878 was affinity purified using an epitope specific to CCT3 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225878于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 61 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. -
序列相似性
Belongs to the TCP-1 chaperonin family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7203 Human
- Entrez Gene: 12462 Mouse
- Entrez Gene: 100172920 Orangutan
- Entrez Gene: 295230 Rat
- Omim: 600114 Human
- SwissProt: P49368 Human
- SwissProt: P80318 Mouse
- SwissProt: Q5NVF9 Orangutan
see all -
别名
- CCT 3 antibody
- CCT gamma antibody
- CCT-gamma antibody
see all
图片
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Western blot - Anti-CCT3 antibody (ab225878)All lanes : Anti-CCT3 antibody (ab225878) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 1 second -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (ab225878)Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).
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Immunoprecipitation - Anti-CCT3 antibody (ab225878)CCT3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225878 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225878 at 0.4 µg/ml.
Lane 1: ab225878 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (ab225878)Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab225878 被引用在 1 文献中.
- Danni X et al. Chaperonin containing TCP1 subunit 3 (CCT3) promotes cisplatin resistance of lung adenocarcinoma cells through targeting the Janus kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) pathway. Bioengineered 12:7335-7347 (2021). PubMed: 34612768