重组Anti-CCT2抗体[EPR4083] (ab109184)
![Western blot - Anti-CCT2 antibody [EPR4083] (ab109184)](https://www.abcam.cn/ps/products/109/ab109184/Images/ab109184-121767-anti-tcp1-beta-antibody-epr4083-western-blot.jpg "Western blot - Anti-CCT2 antibody [EPR4083] (ab109184)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4083] to CCT2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CCT2抗体[EPR4083]
参阅全部 CCT2 一抗 -
描述
兔单克隆抗体[EPR4083] to CCT2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, MCF-7 and A431 cell lysates; Human kidney tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4083 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
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| WB |
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
1/100 - 1/250. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. -
序列相似性
Belongs to the TCP-1 chaperonin family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10576 Human
- Entrez Gene: 12461 Mouse
- Entrez Gene: 299809 Rat
- Omim: 605139 Human
- SwissProt: P78371 Human
- SwissProt: P80314 Mouse
- SwissProt: Q5XIM9 Rat
- Unigene: 189772 Human
see all -
别名
- 99D8.1 antibody
- CCT 2 antibody
- CCT beta antibody
see all
图片
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Western blot - Anti-CCT2 antibody [EPR4083] (ab109184)All lanes : Anti-CCT2 antibody [EPR4083] (ab109184) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (ab109184)](https://www.abcam.cn/ps/products/109/ab109184/Images/ab109184-261325-anti-tcp1-beta-antibody-epr4083-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (ab109184)Immunofluorescence staining of HeLa cells with purified ab109184 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (ab109184)](https://www.abcam.cn/ps/products/109/ab109184/Images/ab109184-121766-anti-tcp1-beta-antibody-epr4083-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (ab109184)ab109184, at 1/100 dilution, staining CCT2 in Human kidney tissue by Immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CCT2 antibody [EPR4083] (ab109184)](https://www.abcam.cn/ps/products/109/ab109184/Images/ab109184-4-benefits-of-recombinant-antibodies.png)
Anti-CCT2 antibody [EPR4083] (ab109184)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab109184 被引用在 3 文献中.
- Cox A et al. Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma. Front Oncol 12:975088 (2022). PubMed: 36185250
- Ghozlan H et al. Chaperonin-Containing TCP1 Complex (CCT) Promotes Breast Cancer Growth Through Correlations With Key Cell Cycle Regulators. Front Oncol 11:663877 (2021). PubMed: 33996588
- Showalter AE et al. Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis. Sci Rep 10:798 (2020). PubMed: 31964905