AB109184

重组Anti-CCT2抗体[EPR4083]

Anti-CCT2 antibody [EPR4083]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal CCT2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

查看别名

99D8.1, CCTB, CCT2, T-complex protein 1 subunit beta, TCP-1-beta, CCT-beta, Chaperonin containing T-complex polypeptide 1 subunit 2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (AB109184)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (AB109184)

Immunofluorescence staining of HeLa cells with purified ab109184 at a working dilution of 1/500 counter-stained with DAPI. The secondary antibody was an Alexa Fluor^® 488 conjugated goat anti-rabbit (ab150077) used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control PBS was used instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (AB109184)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (AB109184)

ab109184 at 1/100 dilution staining CCT2 in Human kidney tissue by Immunohistochemistry

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CCT2 antibody [EPR4083] (AB109184)

All lanes:

Western blot - Anti-CCT2 antibody [EPR4083] (ab109184) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Predicted band size: 57 kDa

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4083

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CCT2 also known as Chaperonin Containing TCP1 Subunit 2 is part of a group of proteins responsible for facilitating the correct folding of other proteins. It functions as a molecular chaperone and has a molecular weight of approximately 58 kDa. CCT2 is expressed in many tissues with high levels detected in testis and brain tissues. This protein is integral to the chaperonin CCT (or TRiC) complex contributing to essential cellular processes through its role in protein homeostasis.

Biological function summary

CCT2 assists in the folding of cytoskeletal proteins like actin and tubulin which are vital for maintaining the structure and function of cells. As part of the CCT complex which consists of eight different subunits CCT2 plays a significant role in ensuring the proper assembly of multi-protein complexes. The activity of CCT2 is important for the prevention of aggregate formation which otherwise leads to cellular stress.

Pathways

CCT2 significantly influences the protein folding and degradation pathways. It participates in the cellular pathways that govern the proteostasis network collaborating with other chaperones to oversee protein quality control. Related proteins interacting with CCT2 within these pathways include other CCT complex components like CCT3 and CCT4 all contributing to maintaining cellular homeostasis and protecting cells from proteotoxic damage.

CCT2 is connected to cancer and neurodegenerative diseases. Altered expression of CCT2 is linked to the progression of tumor growth interacting with proteins involved in cell cycle regulation and signaling such as p53. Additionally deficits in CCT2 function can exacerbate protein misfolding disorders like Alzheimer's disease where proteins like tau become aggregated contributing to the pathology. Efforts to understand CCT2's role in these conditions highlight the importance of protein homeostasis in maintaining health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).

See full target information CCT2

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1058 PubMed40500564

2025

IGF1/IGF1R signaling promotes the expansion of liver CSCs and serves as a potential therapeutic target in HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuju Tian,Qun Zhang,Bin Wang,Yanzhi Qian,Fengchao Liu

Frontiers in oncology 12:975088 PubMed36185250

2022

Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Cox,Daniel Nierenberg,Oscar Camargo,Eunkyung Lee,Amr S Khaled,Joseph Mazar,Rebecca J Boohaker,Tamarah J Westmoreland,Annette R Khaled

Frontiers in oncology 11:663877 PubMed33996588

2021

Chaperonin-Containing TCP1 Complex (CCT) Promotes Breast Cancer Growth Through Correlations With Key Cell Cycle Regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Ghozlan,Adrian Showalter,Eunkyung Lee,Xiang Zhu,Annette R Khaled

Scientific reports 10:798 PubMed31964905

2020

Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anne E Showalter,Ana C Martini,Daniel Nierenberg,Kristen Hosang,Naima Ahmed Fahmi,Priya Gopalan,Amr S Khaled,Wei Zhang,Annette R Khaled

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-CCT2抗体[EPR4083]

Anti-CCT2 antibody [EPR4083]

Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (AB109184)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (AB109184)

Western blot - Anti-CCT2 antibody [EPR4083] (AB109184)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (4)

Abcam Product Promise

重组Anti-CCT2抗体[EPR4083]

Anti-CCT2 antibody [EPR4083]

Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (AB109184)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (AB109184)

Western blot - Anti-CCT2 antibody [EPR4083] (AB109184)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

替代产品

Primary Antibodies

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (4)

Abcam Product Promise