Anti-CCR9抗体[7E7] (ab52590)
![Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (ab52590)](https://www.abcam.cn/ps/products/52/ab52590/Images/ab52590-169994-anti-ccr9-antibody-7e7-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (ab52590)")
Key features and details
- Rat monoclonal [7E7] to CCR9
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-CCR9抗体[7E7]
参阅全部 CCR9 一抗 -
描述
大鼠单克隆抗体[7E7] to CCR9 -
宿主
Rat -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide:
PTDFTSPIPNMADDYGSEST
, corresponding to N terminal amino acids 3-22 of Mouse CCR9 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 5% Fetal calf serum -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
7E7 -
骨髓瘤
P3x63-Ag8.653 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52590于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/20.
|
|
| Flow Cyt |
1/20.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ICC/IF
1/20. |
|
Flow Cyt
1/20. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
组织特异性
Highly expressed in the thymus and low in lymph nodes and spleen. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10803 Human
- Entrez Gene: 12769 Mouse
- Omim: 604738 Human
- SwissProt: P51686 Human
- SwissProt: Q9WUT7 Mouse
- Unigene: 225946 Human
- Unigene: 440604 Mouse
- Unigene: 442383 Mouse
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别名
- C C chemokine receptor D6 antibody
- C C chemokine receptor type 9 antibody
- C C CKR 9 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (ab52590)
ab52590 staining CCR9 in Mouse brain tissue sections by Immunocytochemistry/ Immunofluorescence.
Red = CCR9
Green = IgA
Blue = CD3
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![Flow Cytometry - Anti-CCR9 antibody [7E7] (ab52590)](https://www.abcam.cn/ps/products/52/ab52590/Images/ab52590-64911-anti-ccr9-antibody-7e7-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR9 antibody [7E7] (ab52590)Overlay histogram showing K562 cells stained with ab52590 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52590, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab52590 被引用在 1 文献中.
- Pabst O et al. Chemokine receptor CCR9 contributes to the localization of plasma cells to the small intestine. J Exp Med 199:411-6 (2004). PubMed: 14744993