Anti-CCR7抗体[4B12] (ab52602)
![Flow Cytometry - Anti-CCR7 antibody [4B12] (ab52602)](https://www.abcam.cn/ps/products/52/ab52602/Images/ab52602-343114-ab52602FLOW1.jpg "Flow Cytometry - Anti-CCR7 antibody [4B12] (ab52602)")
Key features and details
- Rat monoclonal [4B12] to CCR7
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
概述
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产品名称
Anti-CCR7抗体[4B12]
参阅全部 CCR7 一抗 -
描述
大鼠单克隆抗体[4B12] to CCR7 -
宿主
Rat -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Mouse splenocytes.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
4B12 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52602于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use a concentration of 5 - 10 µg/ml.
ab18541 - Rat IgG2b monoclonal, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use a concentration of 5 - 10 µg/ml. ab18541 - Rat IgG2b monoclonal, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
组织特异性
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12775 Mouse
- SwissProt: P47774 Mouse
- Unigene: 2932 Mouse
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别名
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
图片
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Flow Cytometry - Anti-CCR7 antibody [4B12] (ab52602)
C57BL6 mouse splenocytes were incubated with Rat IgG2b, kappa monoclonal [RTK4530] (ab18541) (left) or anti-CCR7 [4B12] antibody (ab52602) (right) at 10ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/2000 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD3ε PerCP (10ug/ml) (ab190299).
Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab52602 被引用在 2 文献中.
- Ritter U et al. Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells. J Leukoc Biol 76:472-6 (2004). PubMed: 15197229
- Ohl L et al. CCR7 governs skin dendritic cell migration under inflammatory and steady-state conditions. Immunity 21:279-88 (2004). PubMed: 15308107
