重组Anti-CCR7抗体[1G8] (ab317369)
![Western blot - Anti-CCR7 Antibody [1G8] (ab317369)](https://www.abcam.cn/ps/products/317/ab317369/Images/ab317369-535938-anti-ccr7-antibody-1g8-western-blot-thymus-spleen-lymph-node-lung-jurkat-macf7-hela-hl60-human-mouse-rat.jpg "Western blot - Anti-CCR7 Antibody [1G8] (ab317369)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [1G8] to CCR7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
概述
-
产品名称
Anti-CCR7抗体[1G8]
参阅全部 CCR7 一抗 -
描述
兔单克隆抗体[1G8] to CCR7 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human CCR7 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P32248 -
阳性对照
- WB: Mouse and rat thymus, spleen, lymph node and lung tissue lysate. PC-12, Daudi, Jurkat, MCF-7, HeLa and HL60 cell lysate. IHC-P: Human spleen and tonsil tissue. ICC/IF: HeLa and MCF-7 cells.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Tris buffered saline, 40% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1G8 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317369于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/300 - 1/5000. Predicted molecular weight: 43 kDa.
|
|
| IHC-P |
1/50 - 1/200.
|
|
| ICC/IF |
1/50 - 1/200.
|
| 说明 |
|---|
|
WB
1/300 - 1/5000. Predicted molecular weight: 43 kDa. |
|
IHC-P
1/50 - 1/200. |
|
ICC/IF
1/50 - 1/200. |
靶标
-
功能
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
组织特异性
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 1236 Human
- Entrez Gene: 12775 Mouse
- Entrez Gene: 287673 Rat
- Omim: 600242 Human
- SwissProt: P32248 Human
- SwissProt: P47774 Mouse
- Unigene: 370036 Human
- Unigene: 2932 Mouse
-
别名
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
图片
-
Western blot - Anti-CCR7 Antibody [1G8] (ab317369)All lanes : Anti-CCR7 Antibody [1G8] (ab317369) at 1/1000 dilution
Lane 1 : Mouse thymus tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse lymph node tissue lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Rat thymus tissue lysate
Lane 6 : Rat spleen tissue lysate
Lane 7 : Rat lymph node tissue lysate
Lane 8 : Rat lung tissue lysate
Lane 9 : Jurkat cell lysate
Lane 10 : MCF-7 cell lysate
Lane 11 : HeLa cell lysate
Lane 12 : HL60 cell lysate
Predicted band size: 43 kDaPrimary antibody AB317369 at 1:1000 dilution and 4degC overnight incubation, followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37degC.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 Antibody [1G8] (ab317369)](https://www.abcam.cn/ps/products/317/ab317369/Images/ab317369-535941-anti-ccr7-antibody-1g8-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 Antibody [1G8] (ab317369)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue incubated with AB317369 at 1:100, overnight at 4degC, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CCR7 Antibody [1G8] (ab317369)](https://www.abcam.cn/ps/products/317/ab317369/Images/ab317369-535942-anti-ccr7-antibody-1g8-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR7 Antibody [1G8] (ab317369)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells staining CCR7 using AB317369 at a 1:100 dilution (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
-
![Western blot - Anti-CCR7 Antibody [1G8] (ab317369)](https://www.abcam.cn/ps/products/317/ab317369/Images/ab317369-535939-anti-ccr7-antibody-1g8-western-blot-mcf7-pc12-daudi-jurkat-human-rat.jpg)
Western blot - Anti-CCR7 Antibody [1G8] (ab317369)All lanes : Anti-CCR7 Antibody [1G8] (ab317369) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : Jurkat cell lysate
Predicted band size: 43 kDaAB317369 at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 Antibody [1G8] (ab317369)](https://www.abcam.cn/ps/products/317/ab317369/Images/ab317369-535940-anti-ccr7-antibody-1g8-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 Antibody [1G8] (ab317369)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue incubated with AB317369 at 1:100, overnight at 4degC, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CCR7 Antibody [1G8] (ab317369)](https://www.abcam.cn/ps/products/317/ab317369/Images/ab317369-535943-anti-ccr7-antibody-1g8--immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR7 Antibody [1G8] (ab317369)Immunocytochemistry/ Immunofluorescence analysis of MCF-7 cells staining CCR7 with AB317369 at a 1:100 dilution (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab317369 尚未被引用在任何文献中。