Anti-CCR5抗体[T21/8] (ab110103)
![Flow Cytometry - Anti-CCR5 antibody [T21/8] (ab110103)](https://www.abcam.cn/ps/products/110/ab110103/Images/ab110103-123209-anti-ccr5-antibody-t21-8-flow-cytometry.jpg "Flow Cytometry - Anti-CCR5 antibody [T21/8] (ab110103)")
Key features and details
- Mouse monoclonal [T21/8] to CCR5
- Suitable for: ELISA, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CCR5抗体[T21/8]
参阅全部 CCR5 一抗 -
描述
小鼠单克隆抗体[T21/8] to CCR5 -
宿主
Mouse -
经测试应用
适用于: ELISA, Flow Cytmore details
不适用于: IHC-P,IP or WB -
种属反应性
与反应: Human -
免疫原
The details of the immunogen for this antibody are not available.
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表位
The antibody reacts with the amino terminus of the protein. The exact amino acids that comprise the epitope recognized by the antibody have not been determined. -
阳性对照
- Human tonsil tissue. Normal Human peripheral blood cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
T21/8 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110103于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 0.5µg for 105-8 cells.
in a final volume of 100 ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
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Flow Cyt
Use 0.5µg for 105-8 cells. in a final volume of 100 ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
应用说明
Is unsuitable for IHC-P,IP or WB.
靶标
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功能
Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. -
组织特异性
Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. -
疾病相关
Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1234 Human
- Omim: 601373 Human
- SwissProt: P51681 Human
- Unigene: 450802 Human
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别名
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
see all
图片
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Flow Cytometry - Anti-CCR5 antibody [T21/8] (ab110103)Staining of normal Human peripheral blood cells with 0.25µg of purified mouse IgG1, K isotype control (open histogram) or 0.25 µg of ab110103 (colored histogram), followed by biotin Anti-mMouse IgG and SAv-PE.
Cells in the lymphocyte gate were used for analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab110103 被引用在 4 文献中.
- Xu J et al. Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression. J Immunother Cancer 11:N/A (2023). PubMed: 37185233
- Yu W et al. miR-4456/CCL3/CCR5 Pathway in the Pathogenesis of Tight Junction Impairment in Chronic Obstructive Pulmonary Disease. Front Pharmacol 12:551839 (2021). PubMed: 33953665
- Shan J et al. Expression and biological functions of the CCL5-CCR5 axis in oral lichen planus. Exp Dermatol 28:816-821 (2019). PubMed: 31006151
- Huang R et al. Loss of Fas expression and high expression of HLA-E promoting the immune escape of early colorectal cancer cells. Oncol Lett 13:3379-3386 (2017). PubMed: 28521443