Anti-CCR5抗体(ab7346)
Key features and details
- Rabbit polyclonal to CCR5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CCR5抗体
参阅全部 CCR5 一抗 -
描述
兔多克隆抗体to CCR5 -
宿主
Rabbit -
特异性
This antibody recognises the N-terminal region of the chemokine receptor CCR5. -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human CCR5 aa 6-20 (N terminal).
Sequence:SSPIYDINYYTSEPC
(Peptide available asab7876) -
阳性对照
- THP-1 whole cell lysate
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常规说明
HIV and chemokine receptorThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 20 µg/ml.
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| WB |
1/1000 - 1/2000. Detects a band of approximately 40 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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IHC-P
Use a concentration of 20 µg/ml. |
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WB
1/1000 - 1/2000. Detects a band of approximately 40 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. -
组织特异性
Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. -
疾病相关
Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1234 Human
- Omim: 601373 Human
- SwissProt: P51681 Human
- Unigene: 450802 Human
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别名
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)
Immunohistochemistry of CCR5 in human lymph node tissue with ab7346 at 2.5 μg/ml.
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Western blot - Anti-CCR5 antibody (ab7346)Anti-CCR5 antibody (ab7346) at 1/1000 dilution + THP-1 whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody (ab7346)Immunofluorescence of CCR5 in human lymph node tissue with ab7346 at 20 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)ab7346 at 20µg/ml staining CCR5 in human lymph node tissue by IHC
数据表及文件
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Datasheet download
文献 (6)
ab7346 被引用在 6 文献中.
- Chengcheng L et al. Identification of the Immune-Related Genes in Tumor Microenvironment That Associated With the Recurrence of Head and Neck Squamous Cell Carcinoma. Front Cell Dev Biol 9:723721 (2021). PubMed: 34490269
- Mikhailova A et al. Antiapoptotic Clone 11-Derived Peptides Induce In Vitro Death of CD4+ T Cells Susceptible to HIV-1 Infection. J Virol 94:N/A (2020). PubMed: 32350074
- Kuzmina A et al. Functional Mimetics of the HIV-1 CCR5 Co-Receptor Displayed on the Surface of Magnetic Liposomes. PLoS One 10:e0144043 (2015). PubMed: 26629902
- Jiang AP et al. Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells. J Virol 90:2928-37 (2015). PubMed: 26719250
- Levina V et al. Role of eotaxin-1 signaling in ovarian cancer. Clin Cancer Res 15:2647-56 (2009). IHC-P ; Human . PubMed: 19351767
- Dittmer A et al. Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10. Cell Mol Life Sci 66:3053-65 (2009). WB ; Human . PubMed: 19603142
