Anti-CCR4抗体[KH-4F5] (ab59550)
![Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)](https://www.abcam.cn/ps/products/59/ab59550/Images/ab59550-73560-anti-ccr4-antibody-kh-4f5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)")
Key features and details
- Mouse monoclonal [KH-4F5] to CCR4
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Recombinant fragment
- Isotype: IgG2a
概述
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产品名称
Anti-CCR4抗体[KH-4F5]
参阅全部 CCR4 一抗 -
描述
小鼠单克隆抗体[KH-4F5] to CCR4 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Synthetic peptide from human CCR4
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.20
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
KH-4F5 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59550于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1.2µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
Use 1µg for 106 cells.
|
| 说明 |
|---|
|
Flow Cyt
Use 1.2µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use 1µg for 106 cells. |
靶标
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功能
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. -
组织特异性
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
细胞定位
Cell membrane. - Information by UniProt
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别名
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [KH-4F5] (ab59550)Spectral Confocal Microscopy of CHO cells using ab59550 at 1 µg/106 cells . CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of ab59550 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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![Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)](https://www.abcam.cn/ps/products/59/ab59550/Images/ab59550-73559-anti-ccr4-antibody-kh-4f5-flow-cytometry.jpg)
Flow Cytometry - Anti-CCR4 antibody [KH-4F5] (ab59550)FACS analysis of BOSC23 cells using ab59550 at 1.2 µg/ 106 cells. BOSC23 cells were transiently transfected with an expression vector encodingeither CCR4 (red curve) or an irrelevant protein(control transfectant). Binding of ab59550 was detected with a PE conjugated secondary antibody.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab59550 被引用在 1 文献中.
- Tian Q et al. The regulatory roles of T helper cells in distinct extracellular matrix characterization in breast cancer. Front Immunol 13:871742 (2022). PubMed: 36159822
