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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)
重组RabMAb

重组Anti-CCR3抗体[Y31] - BSA and Azide free (ab271832)

  • Datasheet
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] - BSA and Azide free (ab271832)
  • Anti-CCR3 antibody [Y31] - BSA and Azide free (ab271832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y31] to CCR3 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Anti-CCR3 antibody [Y31] (ab32512)

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概述

  • 产品名称

    Anti-CCR3抗体[Y31] - BSA and Azide free
    参阅全部 CCR3 一抗
  • 描述

    兔单克隆抗体[Y31] to CCR3 - BSA and Azide free
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF,IP or WB
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Macaque monkey
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • K562 and Daudi cell lysates and human liver tissue.
  • 常规说明

    ab271832 is the carrier-free version of ab32512.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    pH: 7.20
    Constituent: PBS
  • 无载体

    是
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    Y31
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related
    • Cell Biology
    • Other Antibodies
    • Oligo verified

相关产品

  • Alternative Versions

    • Anti-CCR3 antibody [Y31] (ab32512)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271832于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

 

说明
IHC-P
Use at an assay dependent concentration.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

 

应用说明
Is unsuitable for Flow Cyt,ICC/IF,IP or WB.

靶标

  • 功能

    Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • 组织特异性

    In eosinophils as well as trace amounts in neutrophils and monocytes.
  • 序列相似性

    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P51677 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1232 Human
    • Entrez Gene: 12771 Mouse
    • Entrez Gene: 117027 Rat
    • Omim: 601268 Human
    • SwissProt: P51677 Human
    • SwissProt: P51678 Mouse
    • SwissProt: O54814 Rat
    • Unigene: 506190 Human
    • Unigene: 9890 Rat
    see all
  • 别名

    • B chemokine receptor antibody
    • b-chemokine receptor antibody
    • C C chemokine receptor type 3 antibody
    • C C CKR 3 antibody
    • C-C chemokine receptor type 3 antibody
    • C-C CKR-3 antibody
    • CC chemokine receptor 3 antibody
    • CC chemokine receptor type 3 antibody
    • CC CKR 3 antibody
    • CC R3 antibody
    • CC-CKR-3 antibody
    • CCCKR3 antibody
    • CCR 3 antibody
    • CCR-3 antibody
    • CCR3 antibody
    • CCR3_HUMAN antibody
    • CD 193 antibody
    • CD193 antibody
    • CD193 antigen antibody
    • Chemokine (C C motif) receptor 3 antibody
    • chemokine (C-C motif) receptor 3 antibody
    • Chemokine (CC) receptor 1 like 2 antibody
    • Chemokine CC motif receptor 3 antibody
    • Chemokine receptor 3 antibody
    • CK R3 antibody
    • CKR 3 antibody
    • CKR3 antibody
    • CMKB R3 antibody
    • CMKBR 3 antibody
    • CMKBR1L2 antibody
    • CMKBR3 antibody
    • Eosinophil CC chemokine receptor 3 antibody
    • Eosinophil eotaxin receptor antibody
    • Eotaxin receptor antibody
    • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
    • MGC102841 antibody
    • MIP1 Alpha RL2 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] - BSA and Azide free (ab271832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] - BSA and Azide free (ab271832)

    Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32512).
  • Anti-CCR3 antibody [Y31] - BSA and Azide free (ab271832)
    Anti-CCR3 antibody [Y31] - BSA and Azide free (ab271832)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

文献 (0)

发表研究结果有使用 ab271832?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab271832 尚未被引用在任何文献中。

客户评价及客户问答

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Mass Cytometry abreview for Anti-CCR3 antibody [Y31] - BSA and Azide free

Below Average
Abreviews
Abreviews
abreview image
Application
Mass Cytometry
Sample
Mouse Tissue sections (Lung mouse)
Specification
Lung mouse
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Tara Sutherland

Verified customer

提交于 Jul 20 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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