重组Anti-CCR2抗体[EPR23494-47] - BSA and Azide free (ab273054)
![Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)](https://www.abcam.cn/ps/products/273/ab273054/Images/ab273054-4-anti-ccr2-antibody-epr2349447-flow-cytometrythp1.jpg "Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23494-47] to CCR2 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-CCR2抗体[EPR23494-47] - BSA and Azide free
参阅全部 CCR2 一抗 -
描述
兔单克隆抗体[EPR23494-47] to CCR2 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cytmore details
不适用于: IHC-P,IP or WB -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- ICC/IF: Human PBMC and THP-1 cells. Flow Cyt: Human PBMC and THP-1 cells.
-
常规说明
ab273054 is the carrier-free version of ab254375.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23494-47 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273054于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
靶标
-
功能
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 729230 Human
- Omim: 601267 Human
- SwissProt: P41597 Human
- Unigene: 511794 Human
- Unigene: 705362 Human
-
别名
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
图片
-
Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cells labelling CCR2 with ab254375 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254375).
-
![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)](https://www.abcam.cn/ps/products/273/ab273054/Images/ab273054-2-anti-ccr2-antibody-epr2349447-immunocytochemistry-thp1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCR2 with ab254375 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in THP-1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254375).
-
![Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)](https://www.abcam.cn/ps/products/273/ab273054/Images/ab273054-3-anti-ccr2-antibody-epr2349447-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CCR2 with ab254375 at 1/600 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Cells were stained with rabbit IgG (Left) or ab254375 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254375).
-
![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)](https://www.abcam.cn/ps/products/273/ab273054/Images/ab273054-1-anti-ccr2-antibody-epr2349447-immunocytochemistry-human-pbmc.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC cells labelling CCR2 with ab254375 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in Human PBMC cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254375).
-
![Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)](https://www.abcam.cn/ps/products/273/ab273054/Images/ab273054-5-benefits-of-recombinant-antibodies.png)
Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
Certificate of Compliance
文献 (0)
ab273054 尚未被引用在任何文献中。
