AB233832

Anti-CCR1抗体[8E10G6]

Name: CCR1抗体[8E10G6] (ab233832)| Abcam中文官网
Brand: Abcam Limited
SKU: AB233832
Price: 4864 CNY
Availability: InStock

Anti-CCR1 antibody [8E10G6]

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(3 Publications)

Mouse Monoclonal CCR1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCR1.

查看别名

CD191, CMKBR1, CMKR1, SCYAR1, CCR1, C-C chemokine receptor type 1, C-C CKR-1, CC-CKR-1, CCR-1, HM145, LD78 receptor, Macrophage inflammatory protein 1-alpha receptor, RANTES-R, MIP-1alpha-R

3 Images

Flow Cytometry - Anti-CCR1 antibody [8E10G6] (AB233832)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR1 antibody [8E10G6] (AB233832)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells labeling CCR1 using ab233832 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-CCR1 antibody [8E10G6] (AB233832)

Expected MW is 37 kDa.

Lane 1:

Western blot - Anti-APAF1 antibody [2H9A1] (<a href='/products/primary-antibodies/apaf1-antibody-2h9a1-ab233786'>ab233786</a>) at 1/500 dilution

Lane 1:

Western blot - Anti-CCR1 antibody [8E10G6] (ab233832) at 1/500 dilution

All lanes:

Recombinant human CCR1 protein

Predicted band size: 141 kDa,41 kDa

true

Supplier Data

Western blot - Anti-CCR1 antibody [8E10G6] (AB233832)

Lanes 1 - 2:

Western blot - Anti-CCR1 antibody [8E10G6] (ab233832) at 1/500 dilution

Lanes 1 - 2:

Western blot - Anti-APAF1 antibody [2H9A1] (<a href='/products/primary-antibodies/apaf1-antibody-2h9a1-ab233786'>ab233786</a>) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

CCR1-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 141 kDa,41 kDa

true

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

8E10G6

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

Flow Cyt, WB

applications

免疫原

Recombinant Fragment Protein within Human CCR1. The exact immunogen used to generate this antibody is proprietary information.

P32246

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

Purified from tissue culture supernatant.

存储溶液

Preservative: 0.05% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The target CCR1 also known as C-C chemokine receptor type 1 plays a mechanical role in mediating cellular responses to chemokines. It is a protein with a molecular mass of approximately 45 kDa. CCR1 expresses on a variety of cells including immune cells like monocytes macrophages and certain lymphocytes as well as some non-hematopoietic cells. This receptor resides on the cell membrane and contributes to the directed movement of these cells in response to chemokine gradients.

Biological function summary

CCR1 involves itself in processes such as leukocyte migration and inflammatory response. The receptor interacts with specific chemokines of the C-C motif like CCL3 and CCL5. CCR1 does not operate within a higher-order protein complex but binds directly to these chemokines leading to signal transduction that alters cell functions. By modulating immune cell behaviors CCR1 helps orchestrate immune surveillance and inflammation.

Pathways

CCR1 plays a significant part in immune-related pathways such as chemokine signaling and the inflammatory response. It transduces signals via G-protein coupled receptor pathways influencing downstream signaling molecules including PI3K and AKT. Apart from its direct chemokine interactions CCR1 regulates functions in concert with proteins like CCR5 another chemokine receptor coordinating the migration and activation of immune cells across tissues.

CCR1 contributes to conditions such as rheumatoid arthritis and multiple sclerosis. Its role in directing immune cell trafficking makes it a potential player in autoimmune inflammation observed in these diseases. CCR1's interaction with other proteins such as CCR5 and their combined roles in these conditions suggest a target for therapeutic intervention. Research continues to explore how modulating CCR1 activity might impact disease progression and symptom severity in affected individuals.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Chemokine receptor that plays a crucial role in regulating immune cell migration, inflammation, and immune responses (PubMed : 14991608). Contributes to the inflammatory response by recruiting immune cells, such as monocytes, macrophages, T-cells, and dendritic cells, to sites of inflammation for the clearance of pathogens and the resolution of tissue damage. When activated by its ligands including CCL3, CCL5-9, CCL13-16 and CCL23, triggers a signaling cascade within immune cells, leading to their migration towards the source of the chemokine (PubMed : 15905581). For example, mediates neutrophil migration after activation by CCL3 leading to the sequential release of TNF-alpha and leukotriene B4 (By similarity). Mediates also monocyte migration upon CXCL4 binding (PubMed : 29930254). Activation by CCL5 results in neuroinflammation through the ERK1/2 signaling pathway (By similarity).

See full target information CCR1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39273021

2024

Cell-Sonar, a Novel Method for Intracellular Tracking of Secretory Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Thomas Seeger,Franz Worek,Simone Rothmiller

Theranostics 14:5123-5140 PubMed39267775

2024

EBV-associated epithelial cancers cells promote vasculogenic mimicry formation via a secretory cross-talk with the immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Xiang,Fengze Sun,Tingting Liu,Jingjing Zhao,Jieying Yang,Dijun Ouyang,Hao Chen,Qian Zhu,Qijing Wang,Yongqiang Li,Jia He,Chaopin Yang,Xinyi Yang,Yuanyuan Chen,Yan Tang,Desheng Weng,Qiuzhong Pan,Qi Yang,Jianchuan Xia

Cell death & disease 13:478 PubMed35589690

2022

Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haixu Xu,Jie Zhao,Jinping Li,Zhifeng Zhu,Zhaohai Cui,Ran Liu,Rong Lu,Zhi Yao,Qiong Xu

View all publications

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Anti-CCR1抗体[8E10G6]

Anti-CCR1 antibody [8E10G6]

Flow Cytometry - Anti-CCR1 antibody [8E10G6] (AB233832)

Western blot - Anti-CCR1 antibody [8E10G6] (AB233832)

Western blot - Anti-CCR1 antibody [8E10G6] (AB233832)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原