Anti-CCNB1IP1抗体(ab71977)
Key features and details
- Mouse polyclonal to CCNB1IP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CCNB1IP1抗体
参阅全部 CCNB1IP1 一抗 -
描述
小鼠多克隆抗体to CCNB1IP1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human CCNB1IP1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- CCNB1IP1 transfected lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71977于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 32 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 32 kDa. |
靶标
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功能
E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis. -
组织特异性
Highly expressed in heart. Detected at intermediate levels in liver and kidney, and at low levels in placenta, brain and lung. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger. -
翻译后修饰
Ubiquitinated; autoubiquitinated.
Phosphorylated by CDK1 on serine or threonine residues (in vitro). -
细胞定位
Nucleus. Chromosome. May associate with segregating chromosomes during metaphase and anaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 57820 Human
- Omim: 608249 Human
- SwissProt: Q9NPC3 Human
- Unigene: 107003 Human
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别名
- C14orf18 antibody
- CCNB1IP1 antibody
- Chromosome 14 open reading frame 18 antibody
see all
图片
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Western blot - Anti-CCNB1IP1 antibody (ab71977)All lanes : Anti-CCNB1IP1 antibody (ab71977) at 1/500 dilution
Lane 1 : CCNB1IP1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 32 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (5)
ab71977 被引用在 5 文献中.
- Palmer N et al. Identification PMS1 and PMS2 as potential meiotic substrates of CDK2 activity. PLoS One 18:e0283590 (2023). PubMed: 36952545
- Gray S et al. Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner. Cell Rep 32:107858 (2020). PubMed: 32640224
- Huang S et al. Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma. Aging (Albany NY) 12:3025-3041 (2020). PubMed: 32045367
- Toledo M et al. A mutation in the endonuclease domain of mouse MLH3 reveals novel roles for MutL? during crossover formation in meiotic prophase I. PLoS Genet 15:e1008177 (2019). PubMed: 31170160
- Hu W et al. MiR-373-3p enhances the chemosensitivity of gemcitabine through cell cycle pathway by targeting CCND2 in pancreatic carcinoma cells. Biomed Pharmacother 105:887-898 (2018). PubMed: 30021382
