Anti-CCM2抗体[OTI2E4] (ab123930)
![Western blot - Anti-CCM2 antibody [OTI2E4] (ab123930)](https://www.abcam.cn/ps/products/123/ab123930/Images/ab123930-137319-anti-ccm2-antibody-oti2e4-western-blot.jpg "Western blot - Anti-CCM2 antibody [OTI2E4] (ab123930)")
Key features and details
- Mouse monoclonal [OTI2E4] to CCM2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-CCM2抗体[OTI2E4]
参阅全部 CCM2 一抗 -
描述
小鼠单克隆抗体[OTI2E4] to CCM2 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human CCM2 aa 1-444. Produced in HEK-293T cells (NP_113631).
Sequence:MEEEGKKGKKPGIVSPFKRVFLKGEKSRDKKAHEKVTERRPLHTVVLSLP ERVEPDRLLSDYIEKEVKYLGQLTSIPGYLNPSSRTEILHFIDNAKRAHQ LPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAA HLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAA ESKVAAEELCCLLGQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDD SSTKVDIKETYEVEASTFCFPESVDVGGASPHSKTISESELSASATELLQ DYMLTLRTKLSSQEIQQFAALLHEYRNGASIHEFCINLRQLYGDSRKFLL LGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRHRRALSTTSSSTT NGNRATGSSDDRSAPSEGDEWDRMISDISSDIEALGCSMDQDSA
Database link: Q9BSQ5 -
阳性对照
- WB: HEK-293T cells transfected with pCMV6-ENTRY CCM2 cDNA. IHC-P: Human kidney tissue. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY CCM2 cDNA.
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常规说明
Clone OTI2E4 (formerly 2E4).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI2E4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab123930于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 49 kDa.
|
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| IHC-P |
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100.
|
| 说明 |
|---|
|
WB
1/2000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/100. |
靶标
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功能
May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock. -
疾病相关
Defects in CCM2 are the cause of cerebral cavernous malformations type 2 (CCM2) [MIM:603284]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. -
序列相似性
Contains 1 PID domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 83605 Human
- Omim: 607929 Human
- SwissProt: Q9BSQ5 Human
- Unigene: 148272 Human
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别名
- C7orf22 antibody
- Ccm2 antibody
- CCM2 gene antibody
see all
图片
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Western blot - Anti-CCM2 antibody [OTI2E4] (ab123930)All lanes : Anti-CCM2 antibody [OTI2E4] (ab123930) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY CCM2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 49 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody [OTI2E4] (ab123930)](https://www.abcam.cn/ps/products/123/ab123930/Images/ab123930-137321-anti-ccm2-antibody-oti2e4-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody [OTI2E4] (ab123930)pCMV6-ENTRY CCM2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for CCM2 using ab123930 at a 1/100 dilution in ICC/IF.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCM2 antibody [OTI2E4] (ab123930)](https://www.abcam.cn/ps/products/123/ab123930/Images/ab123930-137320-anti-ccm2-antibody-oti2e4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCM2 antibody [OTI2E4] (ab123930)Paraffin-embedded human kidney tissue stained for CCM2 with ab123930 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab123930 被引用在 1 文献中.
- Costa B et al. STK25 Protein Mediates TrkA and CCM2 Protein-dependent Death in Pediatric Tumor Cells of Neural Origin. J Biol Chem 287:29285-9 (2012). PubMed: 22782892