重组Anti-CCL4/MIP-1 beta抗体[RP23040078]

• IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-CCL4/MIP-1 beta antibody [RP23040078] (AB308044)

Immunohistochemical analysis of paraffin-embedded human brain tissue labeling CCL4/MIP-1 beta with ab308044 at 1/20 dilution in 3% BSA-PBS followed by HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit (right). The section was incubated with ab308044 for 1 hour at 37ºC in a humidified chamber. Counter stained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Secondary antibody only control (left) : Primary diluent was used instead of primary antibody, followed by HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit

• IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-CCL4/MIP-1 beta antibody [RP23040078] (AB308044)

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling CCL4/MIP-1 beta with ab308044 at 1/20 dilution in 3% BSA-PBS followed by HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit (right). The section was incubated with ab308044 for 1 hour at 37ºC in a humidified chamber. Counter stained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Secondary antibody only control (left) : Primary diluent was used instead of primary antibody, followed by HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit

• WB

Supplier Data

Western blot - Anti-CCL4/MIP-1 beta antibody [RP23040078] (AB308044)

Western blot analysis of CCL4/MIP-1-beta in whole cell extracts from Jurkat cells (lane 1), and K562 cells (lane 2) using ab308044 at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~15kDa.

All lanes:

Western blot - Anti-CCL4/MIP-1 beta antibody [RP23040078] (ab308044) at 1 µg/mL

Lane 1:

Jurkat cells whole cell extracts

Lane 2:

K562 cells whole cell extracts

Observed band size: 15 kDa

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