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AB259496

重组Anti-CCL25抗体[EPR21835-289] - BSA and Azide free (Detector)

Anti-CCL25 antibody [EPR21835-289] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CCL25 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

SCYA25, TECK, CCL25, C-C motif chemokine 25, Chemokine TECK, Small-inducible cytokine A25, Thymus-expressed chemokine

1 Images
Sandwich ELISA - Anti-CCL25 antibody [EPR21835-289] - BSA and Azide free (Detector) (AB259496)
  • sELISA

Unknown

Sandwich ELISA - Anti-CCL25 antibody [EPR21835-289] - BSA and Azide free (Detector) (AB259496)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab242251).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21835-289

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB242251

Human TECK ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human TECK ELISA Kit (AB242251)

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Sample Prep & Detection Kits

AB188288

Cy5® Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Cy5® Conjugation Kit (Fast) - Lightning-Link® (AB188288)

  • 5
  • 1
Sample Prep & Detection Kits

AB270703

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (AB270703)

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Primary Antibodies

AB319067

Anti-IL-16 antibody [EPR28016-53] - BSA and Azide free (Capture)

Recombinant|RabMAb

Sandwich ELISA - Anti-IL-16 antibody [EPR28016-53] - BSA and Azide free (Capture) (AB319067)

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Primary Antibodies

AB270344

Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-57] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-57] - BSA and Azide free (Capture) (AB270344)

  • 0
  • 0
Primary Antibodies

AB278327

Anti-CCL1 antibody [EPR24171-79] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CCL1 antibody [EPR24171-79] - BSA and Azide free (Detector) (AB278327)

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Primary Antibodies

AB259459

Anti-LIF antibody [EPR21949-30] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-LIF antibody [EPR21949-30] - BSA and Azide free (Capture) (AB259459)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab259496 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TECK. The recommended pair for sandwich ELISA is:
Capture: ab259495, Human TECK Capture Antibody (unconjugated)
Detector: ab259496, Human TECK Detector Antibody (unconjugated)
The reference range value is 23.44 - 1500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CCL25 also known as TECK stands as a chemokine with a central role in immune cell attraction. It weighs approximately 15 kDa and is mainly expressed in the thymus and small intestine. This chemokine's expression is associated with its activity in guiding the movement of various immune cells like lymphocytes. Additionally CCL25 interacts with its receptor CCR9 facilitating important cellular signaling necessary for immune responses.
Biological function summary

This chemokine aids in the migration and homing of T-cell subsets particularly to the gut and small intestine hinting at its role in mucosal immunity. CCL25 does not belong to a larger multiprotein complex but it plays a standalone role in controlling immune surveillance and response. Its involvement contributes to the precise regulation of immune cell localization especially under inflammatory conditions.

Pathways

CCL25 participates significantly in the chemokine signaling pathway and the integrin-mediated adhesion pathway. It cooperates with proteins like CCR9 which is its receptor to activate downstream signaling cascades necessary for immune cell trafficking and adhesion. Through these pathways CCL25 ensures correct immune cell distribution throughout the body supporting effective immune surveillance and response.

CCL25 is associated with inflammatory bowel disease (IBD) and celiac disease. In these conditions CCL25 together with its receptor CCR9 contributes to the recruitment of lymphocytes to the inflamed gut. This recruitment exacerbates inflammation and may also link to other inflammatory proteins like TNF-alpha fostering an environment of sustained inflammation. Understanding CCL25's role in such diseases could inform therapeutic strategies aimed at modulating immune cell trafficking.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
See full target information CCL25
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Abcam Product Promise

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