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重组RabMAb

重组Anti-CCL21抗体[EPR6218] (ab129014)

  • Datasheet
  • SDS
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Western blot - Anti-CCL21 antibody [EPR6218] (ab129014)
  • Anti-CCL21 antibody [EPR6218] (ab129014)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6218] to CCL21
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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概述

  • 产品名称

    Anti-CCL21抗体[EPR6218]
    参阅全部 CCL21 一抗
  • 描述

    兔单克隆抗体[EPR6218] to CCL21
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
    不适用于: ICC/IF,IHC-P or IP
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide within Human CCL21 aa 50-150. The exact sequence is proprietary.

  • 阳性对照

    • Recombinant Human CCL21 protein; Caco 2 cell lysate.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR6218
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)

相关产品

  • Alternative Versions

    • Anti-CCL21 antibody [EPR6218] - BSA and Azide free (ab248260)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab129014于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 15 kDa).
说明
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 15 kDa).
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.
  • 组织特异性

    Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.
  • 序列相似性

    Belongs to the intercrine beta (chemokine CC) family.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession O00585 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6366 Human
    • Omim: 602737 Human
    • SwissProt: O00585 Human
    • Unigene: 57907 Human
    • 别名

      • 6Ckine antibody
      • Beta chemokine exodus 2 antibody
      • Beta-chemokine exodus-2 antibody
      • C C motif chemokine ligand 21 antibody
      • C-C motif chemokine 21 antibody
      • CCL21 antibody
      • CCL21_HUMAN antibody
      • Chemokine (C-C motif) ligand 21 antibody
      • Chemokine CC motif ligand 21 antibody
      • CKb9 antibody
      • ECL antibody
      • Efficient Chemoattractant for Lymphocytes antibody
      • SCYA21 antibody
      • Secondary lymphoid tissue chemokine antibody
      • Secondary lymphoid-tissue chemokine antibody
      • SLC antibody
      • Small inducible cytokine A21 antibody
      • Small inducible cytokine subfamily A (Cys-Cys), member 21 antibody
      • Small-inducible cytokine A21 antibody
      • TCA4 antibody
      • UNQ784/PRO1600 antibody
      see all

    图片

    • Western blot - Anti-CCL21 antibody [EPR6218] (ab129014)
      Western blot - Anti-CCL21 antibody [EPR6218] (ab129014)
      All lanes : Anti-CCL21 antibody [EPR6218] (ab129014) at 1/1000 dilution

      Lane 1 : Human CCL21 recombinant protein at 0.01 µg
      Lane 2 : Caco 2 cell lysate at 10 µg

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 15 kDa

    • Anti-CCL21 antibody [EPR6218] (ab129014)
      Anti-CCL21 antibody [EPR6218] (ab129014)

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab129014?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab129014 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    Do you have a mouse monoclonal antibody to CCL21 that has been tested? I noticed on your website that there was one polyclonal antibody available, but has it been verified to work with cell lysates? If not, would it be possible to get a small aliquot to test?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 18 2012

    Answer

    We currently have six anti-CCL21 antibodies in our catalog. Of these, four are polyclonal (3 rabbit, 1 chicken), one is mouse monoclonal (ab89396) and one is rabbit monoclonal (ab129014). Our mouse monoclonal antibody, ab89396 has, to our knowledge, only been tested with recominent fragement. Our rabbit monoclonal, ab129014, has been tested against Caco 2 cells. More information about this product may be found at: https://www.abcam.com/CCL21-antibody-EPR6218-ab129014.html

    Read More

    Abcam Scientific Support

    回复于 Dec 18 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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