AB280371

重组Anti-CCL1抗体[EPR21168-154] - BSA and Azide free

Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CCL1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

查看别名

SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

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Supplier Data

Immunoprecipitation - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (AB280371)

This data was developed using ab280356, the same antibody clone in a different buffer formulation.

CCL1 was immunoprecipitated from 0.35 mg Jurkat (human t cell leukemia t lymphocyte) treated with 10ng/ml PMA and 1μg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate 10 μg with ab280356 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Jurkat treated with 10ng/ml PMA and 1μg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate 10 μg

Lane 2 : ab280356 IP in Jurkat treated with 10ng/ml PMA and 1μg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab280356 in Jurkat treated with 10ng/ml PMA and 1μg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 76 seconds

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-CCL1 antibody [EPR21168-154] (<a href='/products/primary-antibodies/ccl1-antibody-epr21168-154-ab280356'>ab280356</a>)

Predicted band size: 10 kDa

Observed band size: 14 kDa

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Lab

Western blot - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (AB280371)

This data was developed using ab280356, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

CCL1 expression is induced by PMA and anti-CD28 antibody (PMID : 22413885).

Exposure time : 37 seconds

All lanes:

Western blot - Anti-CCL1 antibody [EPR21168-154] (<a href='/products/primary-antibodies/ccl1-antibody-epr21168-154-ab280356'>ab280356</a>) at 1/1000 dilution

Lane 1:

Untreated Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 40 µg

Lane 2:

Jurkat treated with /ml PMA and 1ug/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

Observed band size: 14 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21168-154

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab280356 is the carrier-free version of ab280371.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The CCL1 protein also known as I-309 protein is a cytokine that plays a significant role in the immune system. Its molecular mass is approximately 10 kDa. CCL1 is secreted by activated T cells and is found extensively in the thymus. It binds to the CCR8 receptor which suggests its involvement in immune signaling. This protein belongs to the CC chemokine family which is characterized by the presence of two adjacent cysteines near the amino terminus.

Biological function summary

CCL1 influences immune cell trafficking and activation. It attracts monocytes T lymphocytes and some types of dendritic cells to sites of inflammation. Although it is not part of a large protein complex CCL1 works with other chemokines to modulate the immune response contributing to both innate and adaptive immune mechanisms. Its expression may increase or decrease depending on the immune needs of the body.

Pathways

Studies show that CCL1 participates in the chemokine signaling pathway and the effector T-cell pathway. Within the chemokine signaling pathway CCL1 interacts with other chemokines like CCL17 and proteins involved in T-cell migration. This pathway helps coordinate the movement of immune cells to sites of inflammation or injury ensuring an effective immune response.

Research links CCL1 to conditions such as asthma and rheumatoid arthritis. In asthma CCL1 levels influence the recruitment of immune cells to the lungs contributing to inflammation and airway hyper-responsiveness. Similarly in rheumatoid arthritis its interaction with CCR8 has been associated with synovial inflammation. These diseases indicate that CCL1’s role in immune cell migration can have pathological consequences when dysregulated.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.

See full target information CCL1

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-CCL1抗体[EPR21168-154] - BSA and Azide free

Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free

Immunoprecipitation - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (AB280371)

Western blot - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (AB280371)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原