AB241608

Anti-CCDC47抗体

Name: CCDC47抗体 (ab241608)| Abcam中文官网
Brand: Abcam Limited
SKU: AB241608
Price: 4644 CNY
Availability: InStock

Anti-CCDC47 antibody

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(2 Publications)

Rabbit Polyclonal CCDC47 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CCDC47 aa 400 to C-terminus.

查看别名

GK001, MSTP041, PSEC0077, CCDC47, PAT complex subunit CCDC47, Calumin, Coiled-coil domain-containing protein 47

2 Images

Supplier Data

Immunoprecipitation - Anti-CCDC47 antibody (AB241608)

CCDC47 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241608 used for IP at 6 μg per reaction. For WB 0.4 μg/ml.

Lane 1 : ab241608 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Chemiluminescence detection : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CCDC47 antibody (ab241608)

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-CCDC47 antibody (AB241608)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CCDC47 antibody (ab241608) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-239T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 56 kDa

false

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human CCDC47 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96A33

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antibody was affinity purified using an epitope specific to CCDC47 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed : 32814900, PubMed : 32820719, PubMed : 36261522). The MPT complex takes over after the SEC61 complex : following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed : 36261522). Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein (By similarity). Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex (By similarity). Involved in the regulation of calcium ion homeostasis in the ER (PubMed : 30401460). Required for proper protein degradation via the ERAD (ER-associated degradation) pathway (PubMed : 25009997). Has an essential role in the maintenance of ER organization during embryogenesis (By similarity).

See full target information CCDC47

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular psychiatry 30:5777-5789 PubMed40913114

2025

Hyperactivity of subicular parvalbumin interneurons drives early amyloid pathology and cognitive deficits in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Bing Chen,Bo Jiang,Kai Zhuang,He Wang,Hui-Min Peng,Bo-Wen Zhong,Wen-Ting Xie,Kai-Wei Chen,Ting-Ting Zou,Ya Wang,Hui-Li Yang,Qingwei Yang,Jiechao Zhou,Li Zhong,Li-Hua Zhang,Jie Zhang

Nature 614:160-167 PubMed36697828

2023

Visualization of translation and protein biogenesis at the ER membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Max Gemmer,Marten L Chaillet,Joyce van Loenhout,Rodrigo Cuevas Arenas,Dimitrios Vismpas,Mariska Gröllers-Mulderij,Fujiet A Koh,Pascal Albanese,Richard A Scheltema,Stuart C Howes,Abhay Kotecha,Juliette Fedry,Friedrich Förster

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-CCDC47抗体

Anti-CCDC47 antibody

Immunoprecipitation - Anti-CCDC47 antibody (AB241608)

Western blot - Anti-CCDC47 antibody (AB241608)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

产品实验方案

靶点信息

文献 (2)

Abcam Product Promise

Anti-CCDC47抗体

Anti-CCDC47 antibody

Immunoprecipitation - Anti-CCDC47 antibody (AB241608)

Western blot - Anti-CCDC47 antibody (AB241608)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

替代产品

Primary Antibodies

搭配使用产品

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

产品实验方案

靶点信息

文献 (2)

Abcam Product Promise