Anti-CCDC40抗体(ab121727)
Key features and details
- Rabbit polyclonal to CCDC40
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CCDC40抗体 -
描述
兔多克隆抗体to CCDC40 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 19-104 (SEGEKEGNNE SHMVSPPEKD DGQKGEEAVG STEHPEEVTT QAEAAIEEGE VETEGEAAVE GEEEAVSYGD AESEEEYYYT ETSSPE) of Human CCDC40.
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阳性对照
- Human urinary bladder tissue; RT4, U251 MG, Human liver and tonsil lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121727于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Required for assembly of dynein regulatory complex (DRC) and inner dynein arm complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Not required for outer dynein arm complexes assembly. Required for axonemal recruitment of CCDC39. -
疾病相关
Defects in CCDC40 are the cause of primary ciliary dyskinesia type 15 (CILD15) [MIM:613808]. A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. -
序列相似性
Belongs to the CCDC40 family. -
细胞定位
Cytoplasm. Cell projection > cilium. Localizes to cytoplasm and motile cilium. - Information by UniProt
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数据库链接
- Entrez Gene: 55036 Human
- Omim: 613799 Human
- SwissProt: Q4G0X9 Human
- Unigene: 202542 Human
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别名
- CCD40_HUMAN antibody
- ccdc 40 antibody
- CCDC40 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CCDC40 antibody (ab121727)Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton (microtubules). Recommended concentration of ab121727 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC40 antibody (ab121727)ab121727 at 1/500 dilution staining CCDC40 in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry. -
Western blot - Anti-CCDC40 antibody (ab121727)All lanes : Anti-CCDC40 antibody (ab121727) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver tissue lysate
Lane 5 : Human Tonsil tissue lysate
Developed using the ECL technique.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121727 被引用在 1 文献中.
- Hartmann H et al. Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Sci Alliance 1:e201800070 (2018). PubMed: 30456350