Anti-CCDC34/L15抗体(ab122396)
Key features and details
- Rabbit polyclonal to CCDC34/L15
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CCDC34/L15抗体 -
描述
兔多克隆抗体to CCDC34/L15 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CCDC34/L15 aa 92-191 (internal sequence).
Sequence:DEEDVDEDAHDSEAKVASLRGMELQGCASTQVESENNQEEQKQVRLPESR LTPWEVWFIGKEKEERDRLQLKALEELNQQLEKRKEMEEREKRKIIAE EK
Database link: Q96HJ3 -
阳性对照
- IHC-P: Human cerebral cortex, testis and Fallopian tube tissue WB: Human cell line RT-4 ICC/IF: Human cell line A-431
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122396于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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疾病相关
Note=A chromosomal aberration involving CCDC34 is found in a patient with hamartoma of the retinal pigment epithelium and retina. Translocation t(11;18) (p13;p11.2). - Information by UniProt
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数据库链接
- Entrez Gene: 91057 Human
- Omim: 612324 Human
- SwissProt: Q96HJ3 Human
- Unigene: 143733 Human
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别名
- CCD34_HUMAN antibody
- Ccdc34 antibody
- Coiled-coil domain-containing protein 34 antibody
see all
图片
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Western blot - Anti-CCDC34/L15 antibody (ab122396)Anti-CCDC34/L15 antibody (ab122396) + Human cell line RT-4
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Immunocytochemistry/ Immunofluorescence - Anti-CCDC34/L15 antibody (ab122396)Immunofluorescent staining of Human cell line A-431 MG shows localization to nucleoli fibrillar center & nuclear membrane. Recommended concentration of ab122396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC34/L15 antibody (ab122396)ab122430, at 1/500, staining CCDC34/L15 in paraffin embedded Human Fallopian tube tissue by immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC34/L15 antibody (ab122396)ab122430, at 1/500, staining CCDC34/L15 in paraffin embedded Human testis tissue by immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC34/L15 antibody (ab122396)ab122430, at 1/500, staining CCDC34/L15 in paraffin embedded Human cerebral cortex tissue by immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab122396 被引用在 6 文献中.
- Lin Z et al. Up-Regulated CCDC34 Contributes to the Proliferation and Metastasis of Hepatocellular Carcinoma. Onco Targets Ther 13:51-60 (2020). PubMed: 32021254
- Hu DD et al. Overexpression of Coiled-Coil Domain-Containing Protein 34 (CCDC34) and its Correlation with Angiogenesis in Esophageal Squamous Cell Carcinoma. Med Sci Monit 24:698-705 (2018). PubMed: 29397026
- Liu LB et al. High Expression of CCDC34 Is Associated with Poor Survival in Cervical Cancer Patients. Med Sci Monit 24:8383-8390 (2018). PubMed: 30458457
- Jin C et al. Over-expression of ASIC1a promotes proliferation via activation of the ß-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget 8:25977-25988 (2017). PubMed: 27462920
- Qi W et al. Expression of Coiled-Coil Domain Containing 34 (CCDC34) and its Prognostic Significance in Pancreatic Adenocarcinoma. Med Sci Monit 23:6012-6018 (2017). PubMed: 29257799
- Gong Y et al. CCDC34 is up-regulated in bladder cancer and regulates bladder cancer cell proliferation, apoptosis and migration. Oncotarget 6:25856-67 (2015). WB, IHC . PubMed: 26312564