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AB191472

重组Anti-CC2D1A抗体[EPR18421]

Anti-CC2D1A antibody [EPR18421]

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(3 Publications)

Rabbit Recombinant Monoclonal CC2D1A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

查看别名

AKI1, LGD2, CC2D1A, Coiled-coil and C2 domain-containing protein 1A, Akt kinase-interacting protein 1, Five prime repressor element under dual repression-binding protein 1, Putative NF-kappa-B-activating protein 023N, FRE under dual repression-binding protein 1, Freud-1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody [EPR18421] (AB191472)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody [EPR18421] (AB191472)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CC2D1A with ab191472 at 1/150 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasm staining on HeLa cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows :
1. ab191472 at 1/150 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-CC2D1A antibody [EPR18421] (AB191472)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CC2D1A antibody [EPR18421] (AB191472)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CC2D1A with ab191472 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472)
  • WB

Supplier Data

Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/20000 dilution

Lane 1:

Hela (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 104 kDa

Observed band size: 130 kDa

false

Exposure time: 5s

Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472)
  • WB

Supplier Data

Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/20000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 104 kDa

Observed band size: 130 kDa

false

Exposure time: 10s

Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472)
  • WB

Supplier Data

Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472)

5% NFDM/TBST : Blocking and diluting buffer.

The band at 67KD is an isoform of CC2D1A.

Rogaeva A and Albert PR. Eur J Neurosci. 2007; 26(4) : 965-974.

All lanes:

Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/5000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 104 kDa

Observed band size: 130 kDa,67 kDa

false

Exposure time: 5s

不同偶联物与剂型 (1)

  • Carrier free

    Anti-CC2D1A antibody [EPR18421] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR18421

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CC2D1A also known as Coiled-Coil and C2 Domain Containing 1A is a protein of approximately 100 kDa. This protein is widely expressed with significant presence in the brain indicating its potential role in neural functions. It possesses domains that enable interactions with other proteins and membranes suggesting a multifunctional mechanical role in cellular processes.
Biological function summary

CC2D1A participates in regulating signal transduction and intracellular communication. It becomes part of a larger signaling complex involved in essential cellular processes including proliferation and migration. In neural cells it influences synaptic signaling pathways impacting learning and memory formation suggesting its significance in cognitive functions.

Pathways

CC2D1A participates in the NF-kB and AKT/mTOR signaling pathways which are vital for regulating cell survival and growth. Through these pathways CC2D1A interacts with proteins such as AKT1 and IKK modulating cellular responses to stress and growth factors. Its role in these pathways places it at an important intersection of survival and developmental signaling.

CC2D1A is linked to mental health conditions including autism spectrum disorders (ASD) and intellectual disabilities. Mutations in CC2D1A disrupt its function in neural pathways which may contribute to the development of these disorders. Additionally it interacts with protein DISC1 which has also been implicated in psychiatric disorders highlighting the complex network of interactions in neurodevelopmental and psychiatric disease pathways.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcription factor that binds specifically to the DRE (dual repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. May play a role in the altered regulation of HTR1A associated with anxiety and major depression. Mediates HDAC-independent repression of HTR1A promoter in neuronal cell. Performs essential function in controlling functional maturation of synapses (By similarity). Plays distinct roles depending on its localization. When cytoplasmic, acts as a scaffold protein in the PI3K/PDK1/AKT pathway. Repressor of HTR1A when nuclear. In the centrosome, regulates spindle pole localization of the cohesin subunit SCC1/RAD21, thereby mediating centriole cohesion during mitosis.
See full target information CC2D1A
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34948116

2021

Effects of a in the Hippocampus on Behavior, the Serotonin System, and BDNF.

Applications

Unspecified application

Species

Unspecified reactive species

Elena M Kondaurova,Alexandra V Plyusnina,Tatiana V Ilchibaeva,Dmitry V Eremin,Alexander Ya Rodnyy,Yulia D Grygoreva,Vladimir S Naumenko

International journal of molecular sciences 21: PubMed33233644

2020

Genetic Background Underlying 5-HT Receptor Functioning Affects the Response to Fluoxetine.

Applications

Unspecified application

Species

Unspecified reactive species

Elena M Kondaurova,Alexander Ya Rodnyy,Tatiana V Ilchibaeva,Anton S Tsybko,Dmitry V Eremin,Yegor V Antonov,Nina K Popova,Vladimir S Naumenko

Molecular metabolism 9:176-186 PubMed29398616

2018

Hypothalamic miR-219 regulates individual metabolic differences in response to diet-induced weight cycling.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Schroeder,Yonat Drori,Yair J Ben-Efraim,Alon Chen
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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