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AB21873

Anti-cbx7抗体

Anti-cbx7 antibody

5

(7 Reviews)

|

(53 Publications)

Rabbit Polyclonal CBX7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 53 publications.

查看别名

Chromobox protein homolog 7, CBX7

3 Images
Western blot - Anti-cbx7 antibody (AB21873)
  • WB

Lab

Western blot - Anti-cbx7 antibody (AB21873)

All lanes:

Western blot - Anti-cbx7 antibody (ab21873) at 1 µg

Lane 1:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 2:

Jurkat nuclear extract lysate (<a href='/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Lane 3:

Western blot - Hep G2 nuclear extract lysate (<a href='/products/tissue-lysates/hep-g2-nuclear-extract-lysate-ab14660'>ab14660</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 28 kDa

Observed band size: 100 kDa,34 kDa,70 kDa

true

Exposure time: 30s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody (AB21873)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody (AB21873)

IHC image of cbx7 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21873, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Western blot - Anti-cbx7 antibody (AB21873)
  • WB

Project1161****

Western blot - Anti-cbx7 antibody (AB21873)

All lanes:

Western blot - Anti-cbx7 antibody (ab21873) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Jurkat nuclear extract lysate (<a href='/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Western blot - Hep G2 nuclear extract lysate (<a href='/products/tissue-lysates/hep-g2-nuclear-extract-lysate-ab14660'>ab14660</a>) at 10 µg

Secondary

All lanes:

Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

Predicted band size: 28 kDa

Observed band size: 100 kDa,34 kDa,70 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Replenishment batches of our polyclonal antibody, ab21873 are tested in WB. Previous batches were additionally validated in IHC-P. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab178411.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CBX7 also known as Chromobox protein homolog 7 is a member of the Polycomb group proteins. It has a molecular mass of approximately 27 kDa. Mechanically CBX7 functions as a transcriptional repressor that interacts with chromatin to maintain the repression of genes involved in development and cell differentiation. The protein is expressed in various tissues with notable levels in embryonic stem cells and certain adult tissues like bone marrow and the immune system.
Biological function summary

The CBX7 protein participates in the complex known as Polycomb Repressive Complex 1 (PRC1). This complex plays a significant role in the maintenance of cellular identity by regulating gene expression profiles important for stem cell pluripotency and differentiation. CBX7 binds to modified histones leading to chromatin compaction and gene silencing instrumental for preserving cell lineage integrity during development and cellular transformation.

Pathways

CBX7 interacts with several components in the cell cycle regulation and epigenetic modification pathways. Within the cell cycle regulation pathway it closely interacts with other Polycomb group proteins to control gene expression that governs cell proliferation and differentiation. In the context of epigenetic pathways CBX7 influences chromatin remodeling by interacting with proteins like BMI1 and RING1 thereby exerting control over the transcriptional landscape required for developmental and adaptive responses.

CBX7 shows a relationship with cancer and developmental disorders. Aberrant expression or mutation of CBX7 has been associated with several cancers including prostate and gastric cancers often connected with the suppression of tumor suppressor genes and cell cycle regulation. In these diseases CBX7 interacts with proteins like E2F6 and MYC which are important for cellular proliferation and growth. Moreover disruptions in CBX7 expression patterns can lead to improper cell differentiation contributing to developmental abnormalities.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Promotes histone H3 trimethylation at 'Lys-9' (H3K9me3). Binds to trimethylated lysine residues in histones, and possibly also other proteins. Regulator of cellular lifespan by maintaining the repression of CDKN2A, but not by inducing telomerase activity.
See full target information Chromobox protein homolog 7
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文献 (53)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 16:233 PubMed38037081

2023

Adipose-derived exosomal miR-421 targets CBX7 and promotes metastatic potential in ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Roslyn Tedja,Michael Millman,Terrence Wong,Alexandra Fox,Hussein Chehade,Meyer Gershater,Nicholas Adzibolosu,Radhika Gogoi,Matthew Anderson,Thomas Rutherford,Zhenggang Zhang,Michael Chopp,Gil Mor,Ayesha B Alvero

Oncology letters 27:14 PubMed38028179

2023

CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Tian,Jinglan Deng,Cailing Ma,Ainipa Miershali,Gulikezi Maimaitirexiati,Qi Yan,Yating Liu,Hatimihan Maimaiti,Yuting Li,Changhui Zhou,Jingqin Ren,Lu Ding,Rong Li

Molecular cell 83:1393-1411.e7 PubMed37030288

2023

PRC2.1- and PRC2.2-specific accessory proteins drive recruitment of different forms of canonical PRC1.

Applications

Unspecified application

Species

Unspecified reactive species

Eleanor Glancy,Cheng Wang,Ellen Tuck,Evan Healy,Simona Amato,Hannah K Neikes,Andrea Mariani,Marlena Mucha,Michiel Vermeulen,Diego Pasini,Adrian P Bracken

Nature structural & molecular biology 29:1000-1010 PubMed36220895

2022

Distinct roles for CKM-Mediator in controlling Polycomb-dependent chromosomal interactions and priming genes for induction.

Applications

Unspecified application

Species

Unspecified reactive species

Emilia Dimitrova,Angelika Feldmann,Robin H van der Weide,Koen D Flach,Anna Lastuvkova,Elzo de Wit,Robert J Klose

Frontiers in cell and developmental biology 10:993525 PubMed36176277

2022

upregulates gene expression in the locus through competitive binding to PcG protein CBX7.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoqi Li,Juanli Qiao,Wanru Ma,Jing Zhou,Liankun Gu,Dajun Deng,Baozhen Zhang

Translational oncology 24:101468 PubMed35843065

2022

CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Dai,Yiqi Liu,Renxian Cao,Jingying Cao

ACS omega 7:716-732 PubMed35036738

2022

Discovery of Potent Peptidomimetic Antagonists for Heterochromatin Protein 1 Family Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsey N Lamb,Sarah N Dishman,Jarod M Waybright,Isabelle A Engelberg,Justin M Rectenwald,Jacqueline L Norris-Drouin,Stephanie H Cholensky,Kenneth H Pearce,Lindsey I James,Stephen V Frye

Frontiers in cell and developmental biology 9:783050 PubMed34977026

2021

PDE4B Induces Epithelial-to-Mesenchymal Transition in Bladder Cancer Cells and Is Transcriptionally Suppressed by CBX7.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengnan Huang,Jiakuan Liu,Jiale Yang,Yilin Yan,Chenkai Yang,Xiao He,Ruimin Huang,Mingyue Tan,Denglong Wu,Jun Yan,Bing Shen

Cell chemical biology 29:555-571.e11 PubMed34715055

2021

Reprogramming CBX8-PRC1 function with a positive allosteric modulator.

Applications

Unspecified application

Species

Unspecified reactive species

Junghyun L Suh,Daniel Bsteh,Bryce Hart,Yibo Si,Tyler M Weaver,Carina Pribitzer,Roy Lau,Shivani Soni,Heather Ogana,Justin M Rectenwald,Jacqueline L Norris,Stephanie H Cholensky,Cari Sagum,Jessica D Umana,Dongxu Li,Brian Hardy,Mark T Bedford,Shannon M Mumenthaler,Heinz-Josef Lenz,Yong-Mi Kim,Gang Greg Wang,Ken H Pearce,Lindsey I James,Dmitri B Kireev,Catherine A Musselman,Stephen V Frye,Oliver Bell

Molecular cell 81:4677-4691.e8 PubMed34637753

2021

A Polycomb domain found in committed cells impairs differentiation when introduced into PRC1 in pluripotent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth S Jaensch,Jinjin Zhu,Jesse C Cochrane,Sharon K Marr,Theresa A Oei,Manashree Damle,Ethan Z McCaslin,Robert E Kingston
View all publications
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