AB279707

重组Anti-Cbx6抗体[EPR23828-108] - BSA and Azide free

Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CBX6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

查看别名

Chromobox protein homolog 6, CBX6

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free (AB279707)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free (AB279707)

This data was developed using ab259848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human cerebrum (PMID : 24260522). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free (AB279707)

This data was developed using ab259848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on the immune cells of human colon. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free (AB279707)

This data was developed using ab259848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human hepatocellular carcinoma (PMID : 28122351). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free (AB279707)

This data was developed using ab259848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free (AB279707)

This data was developed using ab259848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on mouse cerebrum. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Lab

Western blot - Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free (AB279707)

This data was developed using ab259848, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Cbx6 antibody [EPR23828-108] (<a href='/products/primary-antibodies/cbx6-antibody-epr23828-108-ab259848'>ab259848</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 2:

Human colon tissue lysate at 40 µg

Lane 3:

Human testis tissue lysate at 40 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 44 kDa

Observed band size: 55 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-Cbx6 antibody [EPR23828-108]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23828-108

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab279707 is the carrier-free version of ab259848.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cbx6 also known as Chromobox Protein Homolog 6 is a component of the Polycomb Group (PcG) proteins with a core function in transcriptional repression. It has a molecular mass of approximately 51 kDa. The primary expression of Cbx6 occurs in stem and progenitor cells but it is also present in various tissues during embryogenesis. The protein contains a chromodomain that facilitates its interaction with histones and contributes to chromatin structure alteration impacting gene expression regulation.

Biological function summary

Cbx6 plays a significant role in maintaining cellular pluripotency and regulating differentiation processes. As part of the Polycomb Repressive Complex 1 (PRC1) Cbx6 participates in modifying chromatin to silence target genes. This complex works synergistically with other PcG proteins to ensure correct temporal and spatial expression of developmentally important genes. The association with histone H3 lysine 27 trimethylation (H3K27me3) is fundamental to its repressive function.

Pathways

Cbx6 functions integrally within the epigenetic regulation pathway and impacts the Wnt signaling pathway. By interacting with other PRC1 members Cbx6 facilitates transcriptional silencing required for the maintenance of stem cell identity. Its activities are closely linked to the modulation of canonical Wnt signaling and in doing so it affects proteins such as β-catenin as well as interacting with other PcG components like Cbx7 and Bmi1.

Cbx6 shows associations with various cancers and developmental disorders. Its dysregulation is observed in malignancies like colorectal cancer where abnormal Cbx6 expression affects the Wnt signaling pathway leading to enhanced proliferation. Furthermore disorders in embryonic development can arise from improper Cbx6 function in Polycomb-mediated gene silencing. Within the context of these conditions Cbx6 works alongside other regulators such as EZH2 in the PRC2 complex highlighting its multifaceted role in disease states.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin (PubMed : 18927235). Recruitment to chromatin might occur in an H3K27me3-independent fashion (By similarity). May have a PRC1-independent function in embryonic stem cells (By similarity).

See full target information CBX6

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Abcam Product Promise

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重组Anti-Cbx6抗体[EPR23828-108] - BSA and Azide free