重组Anti-CBX1 / HP1 beta抗体[RAB-C145] (ab317723)
![Flow Cytometry - Anti-CBX1 / HP1 beta antibody [RAB-C145] (ab317723)](https://www.abcam.cn/ps/products/317/ab317723/Images/ab317723-3-anti-cbx1-hp1-beta-antibody-rab-c145-flow-cytometry-hela-human.jpg "Flow Cytometry - Anti-CBX1 / HP1 beta antibody [RAB-C145] (ab317723)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [RAB-C145] to CBX1 / HP1 beta
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Human
概述
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产品名称
Anti-CBX1 / HP1 beta抗体[RAB-C145]
参阅全部 CBX1 / HP1 beta 一抗 -
描述
人单克隆抗体[RAB-C145] to CBX1 / HP1 beta -
宿主
Human -
特异性
This antibody recognizes CBX1 (Chromobox protein homolog 1). It binds to a folded domain, amino acids 20-73.
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经测试应用
适用于: Flow Cyt, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human CBX1/ HP1 beta aa 1-100. N-terminal tag
Database link: P83916 -
阳性对照
- Flow Cyt: HeLa cells. WB: Nuclear lysates of HeLa, HEK293 and MCF7. ICC/IF: HeLa cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified by Immobilized Metal Affinity Chromatography. -
克隆
单克隆 -
克隆编号
RAB-C145 -
同种型
unknown -
轻链类型
kappa -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317723于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
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| 说明 |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
靶标
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功能
Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. -
组织特异性
Expressed in all adult and embryonic tissues. -
序列相似性
Contains 2 chromo domains. -
翻译后修饰
Not phosphorylated.
Ubiquitinated. -
细胞定位
Nucleus. Unassociated with chromosomes during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 10951 Human
- Omim: 604511 Human
- SwissProt: P83916 Human
- Unigene: 77254 Human
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别名
- CBX 1 antibody
- CBX antibody
- Cbx1 antibody
see all
图片
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Flow Cytometry - Anti-CBX1 / HP1 beta antibody [RAB-C145] (ab317723)
HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of ab317723 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000.
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![Western blot - Anti-CBX1 / HP1 beta antibody [RAB-C145] (ab317723)](https://www.abcam.cn/ps/products/317/ab317723/Images/ab317723-2-anti-cbx1-hp1-beta-antibody-rab-c145-western-blot-hela-hek293-mcf7-human.jpg)
Western blot - Anti-CBX1 / HP1 beta antibody [RAB-C145] (ab317723)Lanes 1-2 : Chimeric rabbit version of ab317723 at 0.3 µg/ml
Lane 3 : Chimeric rabbit version of ab317723 at 0.1 µg/ml
Lane 1 : Nuclear lysates of HeLa (35ug protein in RIPA buffer)
Lane 2 : Nuclear lysates of HEK293 (35ug protein in RIPA buffer)
Lane 3 : Nuclear lysates of MCF7 (35ug protein in RIPA buffer)
Secondary
All lanes : Anti-rabbit secondary antibody.
Predicted band size: 21 kDaA primary incubation of 1h was used and protein was detected by chemiluminescence.
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![Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [RAB-C145] (ab317723)](https://www.abcam.cn/ps/products/317/ab317723/Images/ab317723-1-anti-cbx1-hp1-beta-antibody-rab-c145-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [RAB-C145] (ab317723)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of ab317723 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom primary antibody/secondary, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317723 尚未被引用在任何文献中。