AB226194

Anti-CBS抗体

Name: CBS抗体 (ab226194)| Abcam中文官网
Brand: Abcam Limited
SKU: AB226194
Price: 4890 CNY
Availability: InStock

Anti-CBS antibody

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(1 Publication)

Rabbit Polyclonal CBS antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CBS aa 1-50.

查看别名

Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody (AB226194)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody (AB226194)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CBS using ab226194 at 1/5000 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-CBS antibody (AB226194)

CBS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226194 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226194 at 0.4 μg/ml.

Lane 1 : ab226194 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-CBS antibody (ab226194)

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-CBS antibody (AB226194)

Cell lysates were prepared in NETN lysis buffer.

All lanes:

Western blot - Anti-CBS antibody (ab226194) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 61 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP, IHC-P

applications

免疫原

Synthetic Peptide within Human CBS aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P35520

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab226194 was affinity purified using an epitope specific to CBS immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cystathionine beta-synthase (CBS) is an enzyme that catalyzes the conversion of homocysteine and serine into cystathionine. It is sometimes referred to as CBS monelyne or cystathionine synthase. CBS is a heme-containing protein with a molecular mass of approximately 63 kDa. It is expressed widely in tissues including the liver brain and kidney. The activity of CBS is regulated by various factors including the availability of cofactors like pyridoxal 5'-phosphate and adenosine triphosphate (ATP).

Biological function summary

The enzyme CBS plays a role in the transsulfuration pathway where it helps in the metabolism of homocysteine. CBS is a part of a larger complex in some tissues where it interacts with other enzymes involved in sulfur amino acid metabolism. This function is important in maintaining cellular sulfur amino acid balance and protecting cells from oxidative stress caused by elevated levels of homocysteine.

Pathways

CBS functions in the transsulfuration pathway which connects the methionine cycle and the synthesis of glutathione. This pathway is essential for detoxification and antioxidant protection. CBS interrelates with other proteins like cystathionine gamma-lyase (CGL) within this pathway. The interplay between CBS and CGL ensures the conversion of homocysteine to cysteine which is a precursor for the synthesis of glutathione.

CBS deficiency is linked to homocystinuria a disorder characterized by high levels of homocysteine in the blood and urine. Symptoms include cardiovascular problems and developmental delays. Furthermore alterations in CBS activity relate to cardiovascular diseases due to hyperhomocysteinemia. Within these conditions CBS interacts with other proteins involved in homocysteine metabolism such as methionine synthase which also contributes to the complexity of these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed : 20506325, PubMed : 23974653, PubMed : 23981774). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).

See full target information CBS

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 11:2222-2233 PubMed32127949

2020

The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Ding,Junrui Hua,Jinpeng He,Dong Lu,Wenjun Wei,Yanan Zhang,Heng Zhou,Liying Zhang,Yongqi Liu,Guangming Zhou,Jufang Wang

View all publications

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Anti-CBS抗体

Anti-CBS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody (AB226194)

Immunoprecipitation - Anti-CBS antibody (AB226194)

Western blot - Anti-CBS antibody (AB226194)