AB180939

重组Anti-CBR3抗体[EPR14129]

Name: CBR3抗体[EPR14129] (ab180939)| Abcam中文官网
Brand: Abcam Limited
SKU: AB180939
Price: 2515 CNY
Availability: InStock
Rating: 1 (1 reviews)

Anti-CBR3 antibody [EPR14129]

RabMAb
Recombinant
了解详情

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal CBR3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

SDR21C2, CBR3, Carbonyl reductase [NADPH] 3, NADPH-dependent carbonyl reductase 3, Quinone reductase CBR3, Short chain dehydrogenase/reductase family 21C member 2

2 Images

Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] (AB180939)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] (AB180939)

Intracellular Flow Cytometry analysis of K562 cells labeling CBR3 using ab180939 at a 1/130 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Supplier Data

Western blot - Anti-CBR3 antibody [EPR14129] (AB180939)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CBR3 antibody [EPR14129] (ab180939) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-CBR3 antibody [EPR14129] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14129

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/130", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme carbonyl reductase 3 commonly known as CBR3 is a member of the short-chain dehydrogenase/reductase superfamily. With a molecular mass of approximately 31 kDa CBR3 catalyzes the reduction of carbonyl compounds into alcohols using NADPH as a cofactor. It is expressed in various tissues including liver kidney and heart where it plays a role in xenobiotic metabolism and the detoxification processes by reducing carbonyl-containing compounds.

Biological function summary

CBR3 operates as a critical component in enzymatic systems that protect against oxidative stress. It reduces reactive carbonyls derived from lipid peroxidation products contributing to cellular antioxidant defenses. Although not typically recognized as part of a large complex its activity often synergizes with other reductases and dehydrogenases in these protective roles. This activity helps to limit the damage from lipid peroxidation and therefore maintain cellular function under oxidative stress conditions.

Pathways

CBR3 plays a significant part in the drug metabolism pathway where it metabolizes various therapeutic drugs and environmental toxins. The enzyme interacts closely with NADPH-dependent cofactors and other reductases like CBR1 facilitating phase I detoxification processes. Additionally CBR3 contributes to the prostaglandin synthesis pathway by converting prostaglandin E2 into less active forms interacting relevantly with enzymes involved in prostaglandin metabolism.

CBR3 has been linked to anthracycline-induced cardiotoxicity and certain forms of cancer. In cardiac tissues CBR3 expression influences susceptibility to damage caused by anthracycline antibiotics which are commonly used in cancer chemotherapy. Variations in the gene encoding CBR3 affect the metabolism and clearance of these drugs potentially exacerbating cardiac damage. In cancer CBR3 along with proteins like CBR1 modulates susceptibility to chemotherapeutic agents impacting drug efficacy and toxicity. Understanding the polymorphisms of CBR3 may optimize therapeutic strategies and prevent adverse effects in susceptible individuals.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the NADPH-dependent reduction of carbonyl compounds to their corresponding alcohols (PubMed : 18493841). Has low NADPH-dependent oxidoreductase activity. Acts on several orthoquinones, acts as well on non-quinone compounds, such as isatin or on the anticancer drug oracin (PubMed : 15537833, PubMed : 18493841, PubMed : 19841672). Best substrates for CBR3 is 1,2- naphthoquinone, hence could play a role in protection against cytotoxicity of exogenous quinones (PubMed : 19841672). Exerts activity toward ortho-quinones but not paraquinones. No endogenous substrate for CBR3 except isatin has been identified (PubMed : 19841672).

See full target information CBR3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell division 19:6 PubMed38374109

2024

Anti-cancer effects of Coix seed extract through KCTD9-mediated ubiquitination of TOP2A in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiuyang Jiang,Xue Li,Chun Zhang,Jiafu Wang,Jin Li

View all publications

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重组Anti-CBR3抗体[EPR14129]