重组Anti-CBL (phospho Y731)抗体[EP973Y] (ab52855)
![Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)](https://www.abcam.cn/ps/products/52/ab52855/Images/ab52855-548459-anti-cbl-phospho-y731-antibody-ep973y-western-blot-human-raji.jpg "Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP973Y] to CBL (phospho Y731)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CBL (phospho Y731)抗体[EP973Y]
参阅全部 CBL 一抗 -
描述
兔单克隆抗体[EP973Y] to CBL (phospho Y731) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Raji, NIH/3T3 and C6 whole cell lysates treated with pervanadate.
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常规说明
This product has switched from a hybridoma to recombinant production method on 18th June 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP973Y -
同种型
IgG -
研究领域
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52855于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 100 kDa).
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 100 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects. -
序列相似性
Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain. -
结构域
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. -
翻译后修饰
Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 867 Human
- Entrez Gene: 12402 Mouse
- Entrez Gene: 500985 Rat
- Omim: 165360 Human
- SwissProt: P22681 Human
- SwissProt: P22682 Mouse
- Unigene: 504096 Human
- Unigene: 266871 Mouse
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别名
- 4732447J05Rik antibody
- C CBL antibody
- Cas Br M (murine) ecotropic retroviral transforming sequence antibody
see all
图片
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Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)All lanes : Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855) at 1/1000 dilution
Lane 1 : Untreated Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg
Lane 2 : Raji treated with 1nM pervanadate for 30mins whole cell lysate at 15 µg
Lane 3 : Raji treated with 1nM pervanadate for 30mins whole cell lysate, then the membrane treated with Alkaline phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)](https://www.abcam.cn/ps/products/52/ab52855/Images/ab52855-548460-anti-cbl-phospho-y731-antibody-ep973y-western-blot-mouse-nih-3t3.jpg)
Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)All lanes : Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 2 : NIH/3T3 treated with 10nM pervanadate for 20mins whole cell lysate at 15 µg
Lane 3 : NIH/3T3 treated with 10nM pervanadate for 20mins whole cell lysate, then the membrane treated with Alkaline phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 120 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
![Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)](https://www.abcam.cn/ps/products/52/ab52855/Images/ab52855-548461-anti-cbl-phospho-y731-antibody-ep973y-western-blot-rat-c6.jpg)
Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)All lanes : Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855) at 1/1000 dilution
Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 2 : C6 treated with 10nM pervanadate for 60mins whole cell lysate at 15 µg
Lane 3 : C6 treated with 10nM pervanadate for 60mins whole cell lysate, then the membrane treated with Alkaline phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab52855 被引用在 5 文献中.
- Lu X et al. CTEN Inhibits Tumor Angiogenesis and Growth by Targeting VEGFA Through Down-Regulation of β-Catenin in Breast Cancer. Technol Cancer Res Treat 20:15330338211045506 (2021). PubMed: 34817293
- Gao YF et al. LncRNA FOXD1-AS1 acts as a potential oncogenic biomarker in glioma. CNS Neurosci Ther 26:66-75 (2020). PubMed: 31102349
- Wang D et al. Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling. Nat Immunol 13:560-8 (2012). PubMed: 22561606
- Lee JO et al. Metformin regulates glucose transporter 4 (GLUT4) translocation through AMP-activated protein kinase (AMPK)-mediated Cbl/CAP signaling in 3T3-L1 preadipocyte cells. J Biol Chem 287:44121-9 (2012). WB ; Mouse . PubMed: 23135276
- Kim HS et al. Synergistic signals for natural cytotoxicity are required to overcome inhibition by c-Cbl ubiquitin ligase. Immunity 32:175-86 (2010). WB ; Human . PubMed: 20189481