Anti-CBL (phospho Y700)抗体(ab131345)
Key features and details
- Rabbit polyclonal to CBL (phospho Y700)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CBL (phospho Y700)抗体
参阅全部 CBL 一抗 -
描述
兔多克隆抗体to CBL (phospho Y700) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cells; HT29 cell extracts, treated with UV; Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131345 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131345于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 100 kDa). Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS and 0.1% Tween 20, at 4C, with gentle shaking, overnight.
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/100 - 1/200.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 100 kDa). Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS and 0.1% Tween 20, at 4C, with gentle shaking, overnight. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects. -
序列相似性
Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain. -
结构域
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. -
翻译后修饰
Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 867 Human
- Entrez Gene: 12402 Mouse
- Entrez Gene: 500985 Rat
- Omim: 165360 Human
- SwissProt: P22681 Human
- SwissProt: P22682 Mouse
- Unigene: 504096 Human
- Unigene: 266871 Mouse
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别名
- 4732447J05Rik antibody
- C CBL antibody
- Cas Br M (murine) ecotropic retroviral transforming sequence antibody
see all
图片
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Western blot - Anti-CBL (phospho Y700) antibody (ab131345)All lanes : Anti-CBL (phospho Y700) antibody (ab131345) at 1/500 dilution
Lane 1 : HT29 cell extracts
Lane 2 : HT29 cell extracts treated with UV
Predicted band size: 100 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CBL (phospho Y700) antibody (ab131345)Immunofluorescent analysis of methanol-fixed HeLa cells labelling CBL with ab131345 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y700) antibody (ab131345)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling CBL with ab131345 at 1/50 dilution, in the absence (left) and presence (right) of the blocking peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab131345 尚未被引用在任何文献中。