重组Anti-CBFb抗体[EPR6322] (ab133600)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6322] to CBFb
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CBFb抗体[EPR6322]
参阅全部 CBFb 一抗 -
描述
兔单克隆抗体[EPR6322] to CBFb -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CBFb aa 100-200. The exact sequence is proprietary.
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阳性对照
- WB: A431, Raji, HeLa, K562, Jurkat, and Raw264.7 cell lysates. Mouse and rat spleen and thymus tissue lysates; ICC/IF: Jurkat cells; Flow Cyt (intra): K562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 7.20 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6322 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133600于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/100 - 1/600.
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Flow Cyt (Intra) |
1/100 - 1/10000.
For unpurified use at 1/190. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/100 - 1/600. |
Flow Cyt (Intra)
1/100 - 1/10000. For unpurified use at 1/190. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. -
疾病相关
Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. -
序列相似性
Belongs to the CBF-beta family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 865 Human
- Entrez Gene: 12400 Mouse
- Entrez Gene: 361391 Rat
- Omim: 121360 Human
- SwissProt: Q13951 Human
- SwissProt: Q08024 Mouse
- Unigene: 460988 Human
- Unigene: 2018 Mouse
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别名
- CBF b antibody
- CBF beta antibody
- CBF-beta antibody
see all
图片
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All lanes : Anti-CBFb antibody [EPR6322] (ab133600) at 1/1000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CBFB knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 150 secondsab133600 was shown to react with CBFb in wild-type A431 cells in western blot. Loss of signal was observed when CBFB knockout sample was used. Membranes were blocked in 2 % BSA in TBS-T (0.1 % Tween®) before incubation with ab133600 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
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Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CBFb with purified ab133600 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells, labeling CBFb with purified ab133600 at 1/100 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-CBFb antibody [EPR6322] (ab133600) at 1/1000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CBFB knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDaLanes 1 - 4: Merged signal (red and green). Green - ab133600 observed at 22 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab133600 was shown to react with CBFb in wild-type A431 cells in western blot with loss of signal observed in CBFB knockout sample. Wild-type and CBFB knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 2% BSA in TBS-T (0.1% Tween®) before incubation with ab133600 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-CBFb antibody [EPR6322] (ab133600) at 1/1000 dilution (Purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 5 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 6 : Mouse spleen lysate
Lane 7 : Mouse thymus lysate
Lane 8 : Rat spleen lysate
Lane 9 : Rat thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDaBlocking/Diluting buffer: 5% NFDM/TBST
Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab133600 被引用在 6 文献中.
- Zheng ZQ et al. VIRMA promotes nasopharyngeal carcinoma, tumorigenesis, and metastasis by upregulation of E2F7 in an m6A-dependent manner. J Biol Chem 299:104677 (2023). PubMed: 37028765
- Ni T et al. Dihydrolycorine Attenuates Cardiac Fibrosis and Dysfunction by Downregulating Runx1 following Myocardial Infarction. Oxid Med Cell Longev 2021:8528239 (2021). PubMed: 34725565
- Bergiers I et al. Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis. Elife 7:N/A (2018). PubMed: 29555020
- Chen H et al. Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae. Molecules 22:N/A (2017). PubMed: 28885587
- Peng Y et al. Lovastatin Inhibits Cancer Stem Cells and Sensitizes to Chemo- and Photodynamic Therapy in Nasopharyngeal Carcinoma. J Cancer 8:1655-1664 (2017). PubMed: 28775785
- Nagatake T et al. Central Role of Core Binding Factor ß2 in Mucosa-Associated Lymphoid Tissue Organogenesis in Mouse. PLoS One 10:e0127460 (2015). IHC-Fr ; Mouse . PubMed: 26001080