重组Anti-CBFb抗体[EPR6321] (ab124693)
![Western blot - Anti-CBFb antibody [EPR6321] (ab124693)](https://www.abcam.cn/ps/products/124/ab124693/Images/ab124693-396480-anti-cbfb-antibody-epr6321-western-blot-a431-wt-a431-cbfb-knockout-k562-hela.jpg "Western blot - Anti-CBFb antibody [EPR6321] (ab124693)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6321] to CBFb
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-CBFb抗体[EPR6321]
参阅全部 CBFb 一抗 -
描述
兔单克隆抗体[EPR6321] to CBFb -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CBFb aa 1-100. The exact sequence is proprietary.
-
阳性对照
- WB: A431, HeLa, K562, Jurkat and HUVEC cell lysates.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.97 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6321 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124693于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
靶标
-
功能
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. -
疾病相关
Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. -
序列相似性
Belongs to the CBF-beta family. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 865 Human
- Omim: 121360 Human
- SwissProt: Q13951 Human
- Unigene: 460988 Human
-
别名
- CBF b antibody
- CBF beta antibody
- CBF-beta antibody
see all
图片
-
Western blot - Anti-CBFb antibody [EPR6321] (ab124693)All lanes : Anti-CBFb antibody [EPR6321] (ab124693) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : CBFB knockout A431 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 24-25 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab124693 observed at 24-25 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab124693 was shown to react with CBFb in wild-type A431 cells in western blot. Loss of signal was observed when CBFB knockout sample was used. Wild-type and CBFB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124693 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-CBFb antibody [EPR6321] (ab124693)](https://www.abcam.cn/ps/products/124/ab124693/Images/ab124693-138470-anti-cbfb-antibody-epr6321-western-blot.jpg)
Western blot - Anti-CBFb antibody [EPR6321] (ab124693)All lanes : Anti-CBFb antibody [EPR6321] (ab124693) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa -
![OI-RD Scanning - Anti-CBFb antibody [EPR6321] (ab124693)](https://www.abcam.cn/ps/products/124/ab124693/Images/ab124693-138469-anti-cbfb-antibody-epr6321.jpg)
OI-RD Scanning - Anti-CBFb antibody [EPR6321] (ab124693) -
![Anti-CBFb antibody [EPR6321] (ab124693)](https://www.abcam.cn/ps/products/124/ab124693/Images/ab124693-5-benefits-of-recombinant-antibodies.png)
Anti-CBFb antibody [EPR6321] (ab124693)
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab124693 被引用在 1 文献中.
- Lee SH et al. RUNX3 methylation drives hypoxia-induced cell proliferation and antiapoptosis in early tumorigenesis. Cell Death Differ 28:1251-1269 (2021). PubMed: 33116296