JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB89034

Anti-CBF抗体

Anti-CBF antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Polyclonal CBF antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CEBPZ.

查看别名

CBF2, CEBPZ, CCAAT/enhancer-binding protein zeta, CCAAT-box-binding transcription factor, CBF, CCAAT-binding factor

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CBF antibody (AB89034)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CBF antibody (AB89034)

ab89034, at 10 μg/ml, staining CBF in Hela cell by Immunofluorescence.

Western blot - Anti-CBF antibody (AB89034)
  • WB

Unknown

Western blot - Anti-CBF antibody (AB89034)

All lanes:

Western blot - Anti-CBF antibody (ab89034) at 1 µg/mL

All lanes:

K-562 cell lysate at 50 µg

Predicted band size: 121 kDa

Observed band size: 131 kDa

false

Western blot - Anti-CBF antibody (AB89034)
  • WB

Unknown

Western blot - Anti-CBF antibody (AB89034)

All lanes:

Western blot - Anti-CBF antibody (ab89034) at 1 µg/mL

Lane 1:

CEBPZ transfected 293T cell lysate at 25 µg

Lane 2:

non transfected 293T cell lysate at 25 µg

Predicted band size: 121 kDa

Observed band size: 250 kDa

false

Western blot - Anti-CBF antibody (AB89034)
  • WB

Unknown

Western blot - Anti-CBF antibody (AB89034)

All lanes:

Western blot - Anti-CBF antibody (ab89034) at 1 µg/mL

All lanes:

NIH/3T3 cell lysate at 50 µg

Predicted band size: 121 kDa

Observed band size: 150 kDa

false

关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

ICC/IF, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human CEBPZ.

Q03701

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Core Binding Factor (CBF) also known sometimes as CBF protein is a transcription factor that plays an important role in the regulation of gene expression. CBF is composed of two subunits: the alpha (CBFα) and beta (CBFβ) subunits. CBFβ does not bind DNA on its own but enhances the DNA binding ability of CBFα. The mass of the CBF complex is not fixed and varies depending on the isoform expressions and modifications. It is commonly expressed in hematopoietic cells and bone tissue where it influences the transcriptional control necessary for cell differentiation and development.
Biological function summary

CBF participates in the regulation of critical transcriptional pathways. It's known primarily for its involvement in the binding of DNA motifs working closely with RUNX family proteins to modulate the transcription of genes pivotal for cell cycle and differentiation. CBF functions within a complex most notably with the RUNX family contributing significantly to hematopoietic development and bone formation. Its regulatory mechanisms have broad implications given its influence on essential cellular functions through transcription regulation.

Pathways

CBF integrates into significant biological pathways. Notably it is involved in the RUNX-mediated pathway which is important for hematopoiesis as well as osteogenesis. Within these pathways CBF interacts closely with RUNX1 and RUNX2 to regulate gene transcription required for normal functioning and developmental processes. The interplay and precise regulation of these proteins within these pathways highlight their importance in maintaining cellular homeostasis and proper lineage specification.

CBF's misregulation is linked with leukemia and bone-related disorders. In leukemia especially acute myeloid leukemia chromosomal translocations affecting RUNX1 or CBFβ subunits result in fusion proteins that can disrupt normal gene regulation leading to unregulated cell proliferation. In bone disorders abnormalities in CBF expression or function can affect RUNX2-related pathways impacting bone density and formation evident in conditions like cleidocranial dysplasia. The association of CBF with these disorders highlights its significance in maintaining not only cellular differentiation but also organismal health.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Stimulates transcription from the HSP70 promoter.
See full target information CEBPZ
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:59 PubMed38229174

2024

Intracellular delivery of Parkin-RING0-based fragments corrects Parkin-induced mitochondrial dysfunction through interaction with SLP-2.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Zanon,Marianna Guida,Alexandros A Lavdas,Corrado Corti,Maria Paulina Castelo Rueda,Alessandro Negro,Peter P Pramstaller,Francisco S Domingues,Andrew A Hicks,Irene Pichler
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB176579

Anti-CBF antibody

Immunoprecipitation - Anti-CBF antibody (AB176579)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB109624

Anti-Hsp105/HSP110 antibody [EPR4576]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] (AB109624)

  • 4
  • 4
Primary Antibodies

AB172614

Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (AB172614)

  • 5
  • 1
Primary Antibodies

AB106626

Anti-ZMYM3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZMYM3 antibody (AB106626)

  • 0
  • 0
Primary Antibodies

AB86093

Anti-DRIM antibody

Immunoprecipitation - Anti-DRIM antibody (AB86093)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com