AB176579

Anti-CBF抗体

Name: CBF抗体 (ab176579)| Abcam中文官网
Brand: Abcam Limited
SKU: AB176579
Price: 4606 CNY
Availability: InStock

Anti-CBF antibody

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal CBF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CEBPZ aa 1000 to C-terminus.

查看别名

CBF2, CEBPZ, CCAAT/enhancer-binding protein zeta, CCAAT-box-binding transcription factor, CBF, CCAAT-binding factor

2 Images

Supplier Data

Immunoprecipitation - Anti-CBF antibody (AB176579)

Detection of CBF in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176579 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab176579 was used at 1 μg/ml. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-CBF antibody (ab176579)

Predicted band size: 121 kDa

false

Supplier Data

Western blot - Anti-CBF antibody (AB176579)

All lanes:

Western blot - Anti-CBF antibody (ab176579) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysates at 50 µg

Lane 2:

HeLa whole cell lysates at 15 µg

Lane 3:

HeLa whole cell lysates at 5 µg

Lane 4:

293T whole cell lysates at 50 µg

Predicted band size: 121 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human CEBPZ aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q03701

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab176579 was affinity purified using an epitope specific to CBF immobilized on solid support.

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Core Binding Factor (CBF) also known sometimes as CBF protein is a transcription factor that plays an important role in the regulation of gene expression. CBF is composed of two subunits: the alpha (CBFα) and beta (CBFβ) subunits. CBFβ does not bind DNA on its own but enhances the DNA binding ability of CBFα. The mass of the CBF complex is not fixed and varies depending on the isoform expressions and modifications. It is commonly expressed in hematopoietic cells and bone tissue where it influences the transcriptional control necessary for cell differentiation and development.

Biological function summary

CBF participates in the regulation of critical transcriptional pathways. It's known primarily for its involvement in the binding of DNA motifs working closely with RUNX family proteins to modulate the transcription of genes pivotal for cell cycle and differentiation. CBF functions within a complex most notably with the RUNX family contributing significantly to hematopoietic development and bone formation. Its regulatory mechanisms have broad implications given its influence on essential cellular functions through transcription regulation.

Pathways

CBF integrates into significant biological pathways. Notably it is involved in the RUNX-mediated pathway which is important for hematopoiesis as well as osteogenesis. Within these pathways CBF interacts closely with RUNX1 and RUNX2 to regulate gene transcription required for normal functioning and developmental processes. The interplay and precise regulation of these proteins within these pathways highlight their importance in maintaining cellular homeostasis and proper lineage specification.

CBF's misregulation is linked with leukemia and bone-related disorders. In leukemia especially acute myeloid leukemia chromosomal translocations affecting RUNX1 or CBFβ subunits result in fusion proteins that can disrupt normal gene regulation leading to unregulated cell proliferation. In bone disorders abnormalities in CBF expression or function can affect RUNX2-related pathways impacting bone density and formation evident in conditions like cleidocranial dysplasia. The association of CBF with these disorders highlights its significance in maintaining not only cellular differentiation but also organismal health.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Stimulates transcription from the HSP70 promoter.

See full target information CEBPZ

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Plant biotechnology journal 22:1402-1416 PubMed38163285

2024

Marked enhancement of the immunogenicity of plant-expressed IgG-Fc fusion proteins by inclusion of cholera toxin non-toxic B subunit within the single polypeptide.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Young Kim,Emil Vergara,Andy Tran,Matthew John Paul,Tae-Ho Kwon,Julian K C Ma,Yong-Suk Jang,Rajko Reljic

Nature 552:126-131 PubMed29186125

2017

Promoter-bound METTL3 maintains myeloid leukaemia by mA-dependent translation control.

Applications

Unspecified application

Species

Unspecified reactive species

Isaia Barbieri,Konstantinos Tzelepis,Luca Pandolfini,Junwei Shi,Gonzalo Millán-Zambrano,Samuel C Robson,Demetrios Aspris,Valentina Migliori,Andrew J Bannister,Namshik Han,Etienne De Braekeleer,Hannes Ponstingl,Alan Hendrick,Christopher R Vakoc,George S Vassiliou,Tony Kouzarides

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CBF抗体

Anti-CBF antibody

Immunoprecipitation - Anti-CBF antibody (AB176579)

Western blot - Anti-CBF antibody (AB176579)