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Signal Transduction Protein Trafficking Organelle Proteins

Anti-Caveolin-3抗体- Caveolae Marker (ab2912)

  • Datasheet
  • SDS
Reviews (11)Q&A (4)References (57)

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Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Immunocytochemistry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Immunoprecipitation - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Flow Cytometry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Immunocytochemistry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

Key features and details

  • Rabbit polyclonal to Caveolin-3 - Caveolae Marker
  • Suitable for: IHC-P, ICC/IF, ICC, IHC-Fr, Flow Cyt, WB, IP
  • Reacts with: Mouse, Rat, Sheep, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (ab173575)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Caveolin-3抗体- Caveolae Marker
    参阅全部 Caveolin-3 一抗
  • 描述

    兔多克隆抗体to Caveolin-3 - Caveolae Marker
  • 宿主

    Rabbit
  • 特异性

    This antibody does not detect caveolin-1 or -2.
  • 经测试应用

    适用于: IHC-P, ICC/IF, ICC, IHC-Fr, Flow Cyt, WB, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Sheep, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse Caveolin-3 aa 1-19.
    Sequence:

    MMTEEHTDLEARIIKDIHC


    (Peptide available as ab4930)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Rat heart and skeletal muscle; Mouse heart and skeletal muscle; L6 cell lysate. ICC: C2C11 and HeLa cells. IHC-P: Mouse lymph node, heart and skeletal muscle tissues. IP: Mouse heart tissue lysate. Flow Cyt: U-87 MG cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antigen affinity chromatography.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Muscle
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • Mouse Caveolin-3 peptide (ab4930)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Caveolin-3 protein (ab114265)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2912于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/100 - 1/200.
ICC/IF
Use at an assay dependent concentration.
ICC
1/20.
IHC-Fr (2)
Use at an assay dependent concentration. PubMed: 21408028
Flow Cyt
Use 3-5µg for 106 cells.
WB (8)
Use a concentration of 1 - 3 µg/ml. Can be blocked with Mouse Caveolin-3 peptide (ab4930).
IP
Use at an assay dependent concentration.
说明
IHC-P
1/100 - 1/200.
ICC/IF
Use at an assay dependent concentration.
ICC
1/20.
IHC-Fr
Use at an assay dependent concentration. PubMed: 21408028
Flow Cyt
Use 3-5µg for 106 cells.
WB
Use a concentration of 1 - 3 µg/ml. Can be blocked with Mouse Caveolin-3 peptide (ab4930).
IP
Use at an assay dependent concentration.

靶标

  • 功能

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.
  • 组织特异性

    Expressed predominantly in muscle.
  • 疾病相关

    Defects in CAV3 are the cause of limb-girdle muscular dystrophy type 1C (LGMD1C) [MIM:607801]. LGMD1C is a myopathy characterized by calf hypertrophy and mild to moderate proximal muscle weakness. LGMD1C inheritance can be autosomal dominant or recessive.
    Defects in CAV3 are a cause of hyperCKmia (HYPCK) [MIM:123320]. It is a disease characterized by persistent elevated levels of serum creatine kinase without muscle weakness.
    Defects in CAV3 are a cause of rippling muscle disease (RMD) [MIM:606072]. RMD is a rare disorder characterized by mechanically triggered contractions of skeletal muscle. In RMD, mechanical stimulation leads to electrically silent muscle contractions that spread to neighboring fibers that cause visible ripples to move over the muscle.
    Defects in CAV3 are a cause of cardiomyopathy familial hypertrophic (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in CAV3 are the cause of long QT syndrome type 9 (LQT9) [MIM:611818]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
    Defects in CAV3 can be a cause of sudden infant death syndrome (SIDS) [MIM:272120]. SIDS is the sudden death of an infant younger than 1 year that remains unexplained after a thorough case investigation, including performance of a complete autopsy, examination of the death scene, and review of clinical history. Pathophysiologic mechanisms for SIDS may include respiratory dysfunction, cardiac dysrhythmias, cardiorespiratory instability, and inborn errors of metabolism, but definitive pathogenic mechanisms precipitating an infant sudden death remain elusive. Long QT syndromes-associated mutations can be responsible for some SIDS cases.
  • 序列相似性

    Belongs to the caveolin family.
  • 细胞定位

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Target information above from: UniProt accession P56539 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 859 Human
    • Entrez Gene: 12391 Mouse
    • Entrez Gene: 29161 Rat
    • Omim: 601253 Human
    • SwissProt: P56539 Human
    • SwissProt: P51637 Mouse
    • SwissProt: P51638 Rat
    • Unigene: 98303 Human
    • 别名

      • CAV3 antibody
      • CAV3_HUMAN antibody
      • Caveolin 3 antibody
      • Caveolin-3 antibody
      • LGMD1C antibody
      • LQT9 antibody
      • M-caveolin antibody
      • MGC126100 antibody
      • MGC126101 antibody
      • MGC126129 antibody
      • OTTHUMP00000115603 antibody
      • OTTHUMP00000207105 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    图片

    • Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      All lanes : Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Lane 1 : Rat heart tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : HEK293 cell lysate
      Lane 4 : Rat skeletal muscle tissue lysate
      Lane 5 : Mouse muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/2500 dilution

      Developed using the ECL technique.

      Observed band size: 17 kDa why is the actual band size different from the predicted?



      Blocked with 5% skimmed milk.

    • Immunocytochemistry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Immunocytochemistry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Immunocytochemistry analysis of Caveolin-3 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) (right panel) or with ab2912 at a dilution of 1/20 overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Caveolin-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Immunohistochemistry was performed on normal biopsies of deparaffinized mouse heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Caveolin-3 ab2912 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      All lanes : Anti-Caveolin-3 antibody - Caveolae Marker (ab2912) at 1/1000 dilution

      Lane 1 : L6 (Rat skeletal muscle cell line) whole cell lysate
      Lane 2 : Mouse skeletal muscle tissue lysate
      Lane 3 : Mouse heart tissue lysate
      Lane 4 : Mouse brain tissue lysate
      Lane 5 : Mouse kidney tissue lysate
      Lane 6 : Rat skeletal muscle tissue lysate
      Lane 7 : Rat heart tissue lysate
      Lane 8 : Rat brain tissue lysate
      Lane 9 : Rat kidney tissue lysate

      Lysates/proteins at 30 µg per lane.

      Observed band size: 17 kDa why is the actual band size different from the predicted?



      Western blot was performed using ab2912 and a ~17 kDa band corresponding to Caveolin 3 was observed. Whole cell extracts (30 µg lysate) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane  by iBlot® 2 Dry Blotting System. The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP using the iBright FL 1000. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.

    • Immunoprecipitation - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Immunoprecipitation - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Caveolin-3 was immunoprecipitated using 5 µg of ab2912 from mouse heart tissue lysate (Lane 3) using the protein A beads. Normal rabbit IgG was used as a isotype control (Lane 2). 10% input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using ab2912 and HRP-conjugated goat anti-rabbit IgG (H+L) at a dilution of 1/2500. Chemiluminescent detection was performed.

    • Flow Cytometry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Flow Cytometry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Flow cytometry analysis of U-87 MG cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with ab2912 (red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

    • Immunocytochemistry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Immunocytochemistry - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Immunocytochemistry analysis of Caveolin-3 in C2C11 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) (right panel) or with ab2912 at a dilution of 1/20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Caveolin-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/100 with a rabbit polyclonal antibody recognizing Caveolin-3 ab2912 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Anti-Caveolin-3 antibody - Caveolae Marker (ab2912) + Rat cardiac muscle tissue lysate
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Immunohistochemistry was performed on normal biopsies of deparaffinized mouse lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Caveolin-3 ab2912 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)
      Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody - Caveolae Marker (ab2912)

      Immunocytochemistry/Immunofluorescence analysis of 70% confluent log phase A-375 cells. Cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Samples were incubated with ab2912 at 1µg/ml in 1% BSA for 3 hours at room temperature and then labelled with Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) at a dilution of 1/2000 for 45 minutes at room temperature (panel a: green). Nuclei (panel b: blue) were stained with DAPI. F-actin (panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

    实验方案

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (57)

    发表研究结果有使用 ab2912?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2912 被引用在 57 文献中.

    • Kim H  et al. Heparin-Mimicking Polymer-Based In Vitro Platform Recapitulates In Vivo Muscle Atrophy Phenotypes. Int J Mol Sci 22:N/A (2021). PubMed: 33801235
    • Tao J  et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
    • Gong J  et al. Caveolin-3 protects diabetic hearts from acute myocardial infarction/reperfusion injury through ß2AR, cAMP/PKA, and BDNF/TrkB signaling pathways. Aging (Albany NY) 12:14300-14313 (2020). PubMed: 32692723
    • Russell JS  et al. Dietary a-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection. Nutrients 12:N/A (2020). PubMed: 32887376
    • Sehirli AO  et al. The effects of resveratrol treatment on caveolin-3 expression and Na+/K+ ATPase activity in rats with isoproterenol-induced myocardial injury. North Clin Istanb 7:313-320 (2020). PubMed: 33043254
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 15 Abreviews or Q&A

    Western blot abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Cardiac)
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Loading amount
    10 µg
    Specification
    Cardiac
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 20 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (heart)
    Permeabilization
    Yes - 0.1% Trition X
    Specification
    heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    提交于 Jul 26 2013

    Western blot abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    HEK
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 26 2013

    Western blot abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (heart)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    50 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 26 2013

    Western blot abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    50 µg
    Specification
    heart
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 26 2013

    Western blot abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (kidney)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 03 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rhesus monkey Tissue sections (Vagina)
    Permeabilization
    No
    Specification
    Vagina
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Rebecca Shaffer

    Verified customer

    提交于 Nov 22 2012

    Western blot abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 23 2012

    Western blot abreview for Anti-Caveolin-3 antibody - Caveolae Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    50 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 28 2011

    Question

    Please send an AbTrial code for testing ab1912 by IHC on sections of rhesus macaque tissue.

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    Abcam community

    Verified customer

    Asked on Oct 22 2012

    Answer

    DISCOUNT CODE: ***
    Expiration date: ***
    Value: $***

    I am very pleased to hear you would like to accept our offer and test ab2912 for IHC of rhesus macaque tissue. To redeem this offer, please submit an Abreview for rhesus IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    回复于 Oct 22 2012

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